Daley E F, Pagotto F, Farber J M
Microbiology Research Division, Banting Research Centre, Ottawa, Canada.
Lett Appl Microbiol. 1995 Apr;20(4):195-8. doi: 10.1111/j.1472-765x.1995.tb00426.x.
Various retail and environmental sponges were tested for inhibitory properties against Listeria species and several other bacterial genera. Sterile sponges, unrinsed and rinsed in sterile distilled water or sterile neutralizing buffer, were placed on seeded plates of tryptic soy agar with 0.6% yeast extract. Plates were incubated at 30 degrees C for 24 h and zones of inhibition measured. The Systems Plus environmental sponge and the Technical Service Consultants Ltd sponge (sTc) proved to be the only sponges which consistently demonstrated no inhibitory properties. Results using scanning electron microscopy showed considerable bacterial attachment to the Systems Plus sponge, further corroborating these findings.
对各种零售用海绵和环境用海绵进行了测试，以检测其对李斯特菌属及其他几个细菌属的抑制特性。将无菌海绵，未冲洗的以及在无菌蒸馏水或无菌中和缓冲液中冲洗过的，放置在含有0.6%酵母提取物的胰蛋白胨大豆琼脂接种平板上。平板在30摄氏度下孵育24小时，并测量抑菌圈。Systems Plus环境海绵和Technical Service Consultants Ltd海绵（sTc）被证明是仅有的始终未表现出抑制特性的海绵。扫描电子显微镜的结果显示，细菌大量附着在Systems Plus海绵上，进一步证实了这些发现。
