Tishchenko G, Bleha M, Skvor J, Bures L
Institute of Macromolecular Chemistry, Academy of Sciences of the Czech Republic.
Bioseparation. 1995 Feb;5(1):19-25.
Separation of the ammonium sulfate precipitated protein fraction of mouse ascitic fluid, containing the specific immunoglobulin (pI 6.7-6.8; molecular weight 180000), from ammonium sulfate was investigated by means of non-traditional dialysis, based on the difference in diffusion rates of small and large molecules through porous membranes. The experiments were carried out in spiral membrane modules equipped with a Neosepta (AM-2 or ACS-SB) anion exchange membrane and a microfiltration membrane (Synpore or Sartorius). To enhance the driving force for penetration of ammonium sulfate and low-molecular-weight components from solution of ascitic protein fraction into water, a counterpressure was imposed on the side of microfiltration membrane. The flow rate, counterpressure and the pore sizes of microfiltration membranes had a significant effect on the separation process, as expected. The type of the anion exchange membrane had only a small effect. This process makes it possible to desalt the immunoglobulin fraction with high purity and yield in a few hours instead of 5 days.
基于小分子和大分子通过多孔膜的扩散速率差异，采用非传统透析法研究了从小鼠腹水液硫酸铵沉淀蛋白组分中分离出特定免疫球蛋白（等电点6.7 - 6.8；分子量180000）与硫酸铵的方法。实验在配备Neosepta（AM - 2或ACS - SB）阴离子交换膜和微滤膜（Synpore或Sartorius）的螺旋膜组件中进行。为增强硫酸铵和低分子量组分从腹水蛋白组分溶液渗透到水中的驱动力，在微滤膜一侧施加反压。正如预期的那样，流速、反压和微滤膜的孔径对分离过程有显著影响。阴离子交换膜的类型影响较小。该方法能够在几小时内而非5天，以高纯度和高收率对免疫球蛋白组分进行脱盐。
