Loftus A, Delisle A L
Department of Microbiology, School of Dentistry, University of Maryland at Baltimore 21201, USA.
Curr Microbiol. 1995 May;30(5):317-21. doi: 10.1007/BF00295508.
Doses of 0.1 to 1.0 micrograms/ml of mitomycin C induced cell lysis of six of eight strains of Actinobacillus actinomycetemcomitans tested. Infectious phages were induced from ATCC strains 43717, 29524, 33384, and 43719; non-plaque-forming, possibly defective phages were induced from ATCC strains 29522 and 29523. No phages were detected in strain FDC 651 or ATCC strain 43718. No correlation between lysogeny and leukotoxin production or serotype of the strains could be established. Gel electrophoresis of phage DNAs indicated that the induced phages were of three types, based on size. By electron microscopy, the phages were found to belong to either morphotype A1 or morphotype B1; no other morphotypes were observed. Curing experiments led to the isolation of nonlysogenic derivatives of two strains, which supported plaque formation by the phages they originally carried. On the basis of our results, lysogeny appears to be widespread in A. actinomycetemcomitans.
浓度为0.1至1.0微克/毫升的丝裂霉素C可诱导所检测的8株伴放线放线杆菌中的6株发生细胞裂解。从美国典型培养物保藏中心（ATCC）菌株43717、29524、33384和43719中诱导出了感染性噬菌体；从ATCC菌株29522和29523中诱导出了不形成噬菌斑、可能有缺陷的噬菌体。在FDC 651菌株或ATCC菌株43718中未检测到噬菌体。无法确定溶原性与菌株的白细胞毒素产生或血清型之间的相关性。噬菌体DNA的凝胶电泳表明，根据大小，诱导出的噬菌体有三种类型。通过电子显微镜观察，发现噬菌体属于形态型A1或形态型B1；未观察到其他形态型。治愈实验导致分离出两株菌株的非溶原性衍生物，这支持了它们最初携带的噬菌体形成噬菌斑。根据我们的结果，溶原性似乎在伴放线放线杆菌中广泛存在。
