Barretto Tinoco E M, Stevens R, Haubek D, Lai C H, Balachandran S, Preus H
Department of Periodontology, Faculty of Dentistry, University of Oslo, Norway.
Eur J Oral Sci. 1997 Aug;105(4):310-7. doi: 10.1111/j.1600-0722.1997.tb00246.x.
Actinobacillus actinomycetemcomitans has been associated with different forms of periodontitis, particularly with localized juvenile periodontitis (LJP). The bacterium possesses several virulence factors which have been shown to interact with the host immune system. Among these factors, leukotoxin, surface antigens (serotype) and bacteriophages have been suggested directly or indirectly to influence the course of infection. However, few studies have been able to show associations between these factors and periodontal disease, alone or in combination. Thus, the purpose of the present study was to investigate possible correlations between periodontal disease status and selected virulence factors (serotype, presence of bacteriophages, and the presence of a 530 bp deletion in the promoter region of the leukotoxin gene). 36 subjects took part in the study. Serotype c was the most frequently found serotype among periodontally affected subjects, although serotypes a and b were also present. 27 out of 36 strains harbored bacteriophages, and there was strong evidence that some of the bacteriophages were different from the previously characterized phi Aa phage. A. actinomycetemcomitans containing the F-fragment phage were more frequently associated with periodontal disease. Five strains, all serotype b, 3 from LJP patients and 2 from healthy subjects, showed a 530-bp deletion in the promoter region of the leukotoxin gene.
伴放线放线杆菌与不同形式的牙周炎有关，尤其是与局限性青少年牙周炎（LJP）相关。该细菌具有多种毒力因子，已证明这些因子可与宿主免疫系统相互作用。在这些因子中，白细胞毒素、表面抗原（血清型）和噬菌体已被直接或间接表明会影响感染进程。然而，很少有研究能够单独或联合显示这些因子与牙周疾病之间的关联。因此，本研究的目的是调查牙周疾病状态与选定的毒力因子（血清型、噬菌体的存在以及白细胞毒素基因启动子区域530 bp缺失的存在）之间可能的相关性。36名受试者参与了该研究。在牙周病患者中，血清型c是最常见的血清型，不过血清型a和b也存在。36株菌株中有27株带有噬菌体，并且有强有力的证据表明其中一些噬菌体与先前鉴定的φAa噬菌体不同。含有F片段噬菌体的伴放线放线杆菌与牙周疾病的关联更为频繁。5株菌株，均为血清型b，3株来自LJP患者，2株来自健康受试者，在白细胞毒素基因启动子区域显示出530 bp的缺失。
