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Nucleic Acids Res. 1981 May 25;9(10):2397-410. doi: 10.1093/nar/9.10.2397.
2
Substrate selection by RNA polymerase from E. coli. The role of ribose and 5'-triphosphate fragments, and nucleotides interaction.大肠杆菌RNA聚合酶的底物选择。核糖和5'-三磷酸片段的作用以及核苷酸间的相互作用。
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Properties of ATP and UTP analogues with P-S-C-5' bonds.
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Steady state kinetic studies of initiation of RNA synthesis on T7 DNA in the presence of rifampicin.在利福平存在的情况下，对T7 DNA上RNA合成起始的稳态动力学研究。
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5
[Kinetics of inhibition by 8-oxy-ATP of the dinucleotide pppApU synthesis catalyzed by Escherichia coli RNA-polymerase on the promoter A1 of phage T7 delta D111 DNA during coupled synthesis of di- and trinucleotides and a limited set of substrates].[在二核苷酸和三核苷酸的偶联合成以及一组有限底物存在的情况下，8-氧代-ATP对大肠杆菌RNA聚合酶催化噬菌体T7 δD111 DNA启动子A1上二核苷酸pppApU合成的抑制动力学]
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6
[Kinetics of inhibition by 8-oxy-GTP and 8-bromo-GTP of Escherichia coli RNA-polymerase synthesis of pppApU dinucleotide on the promotor a1 of phage T7deltaD111 DNA in a limited set of substrates].[在一组有限的底物中，8-氧代-GTP和8-溴代-GTP对噬菌体T7δD111 DNA启动子a1上大肠杆菌RNA聚合酶合成三磷酸腺苷-尿苷二核苷酸的抑制动力学]
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7
[Effect of 8-Br-ATP and 8-Oxy-ATP on RNA synthesis by RNA polymerase from Escherichia coli].[8-溴-ATP和8-氧代-ATP对大肠杆菌RNA聚合酶RNA合成的影响]
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Spin-labeled nucleotide substrates for DNA-dependent RNA polymerase from Escherichia coli.用于大肠杆菌DNA依赖性RNA聚合酶的自旋标记核苷酸底物。
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UTP allosterically regulates transcription by Escherichia coli RNA polymerase from the bacteriophage T7 A1 promoter.尿苷三磷酸（UTP）对来自噬菌体T7 A1启动子的大肠杆菌RNA聚合酶的转录具有变构调节作用。
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RNA-protein crosslinking to AMP residues at internal positions in RNA with a new photocrosslinking ATP analog.利用一种新型光交联ATP类似物使RNA与RNA内部位置的AMP残基发生RNA-蛋白质交联。
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本文引用的文献

1
Crystal and molecular structure of 8-bromoguanosine and 8-bromoadenosine, two purine nucleosides in the syn conformation.8-溴鸟苷和8-溴腺苷（两种处于顺式构象的嘌呤核苷）的晶体结构和分子结构
J Mol Biol. 1970 Feb 28;48(1):109-23. doi: 10.1016/0022-2836(70)90222-6.
2
Analysis of transcription in vitro using purine nucleotide analogs.使用嘌呤核苷酸类似物进行体外转录分析。
Biochemistry. 1971 Apr 27;10(9):1525-31. doi: 10.1021/bi00785a003.
3
The relationship of mono- and polynucleotide conformation to catalysis by polynucleotide phosphorylase.单核苷酸和多核苷酸构象与多核苷酸磷酸化酶催化作用的关系。
Biochim Biophys Acta. 1970 Sep 17;217(1):18-29. doi: 10.1016/0005-2787(70)90118-8.
4
A new method of large scale preparation of highly purified DNA-dependent RNA-polymerase from E. coli.一种从大肠杆菌大规模制备高纯度依赖DNA的RNA聚合酶的新方法。
Hoppe Seylers Z Physiol Chem. 1970 Feb;351(2):221-4. doi: 10.1515/bchm2.1970.351.1.221.
5
Poly(2-methyl-N6-methyladenylic acid): synthesis, properties, and interaction with poly(uridylic acid).聚（2-甲基-N6-甲基腺苷酸）：合成、性质及其与聚（尿苷酸）的相互作用。
Biochemistry. 1974 Jun 18;13(13):2754-61. doi: 10.1021/bi00710a015.
6
6-Azauridine-5'-phosphoric acid: unusual molecular structure and functional mechanism.6-氮杂尿苷-5'-磷酸：独特的分子结构与作用机制。
Nature. 1973 Apr 27;242(5400):610-2. doi: 10.1038/242610a0.
7
Some stereochemical requirements of Escherichia coli ribonucleic acid polymerase. Interaction with conformationally restricted ribonucleoside 5'-triphosphates: 8-bromoguanosine, 8-ketoguanosine, and 6-methylcytidine triphosphates.大肠杆菌核糖核酸聚合酶的一些立体化学要求。与构象受限的核糖核苷5'-三磷酸的相互作用：8-溴鸟苷、8-酮鸟苷和6-甲基胞苷三磷酸。
Biochemistry. 1971 Oct 26;10(22):4050-61. doi: 10.1021/bi00798a007.
8
Properties of ATP and UTP analogues with P-S-C-5' bonds.
Eur J Biochem. 1975 Jan 2;50(2):343-9. doi: 10.1111/j.1432-1033.1975.tb09809.x.
9
Phenylalanyl-tRNA and seryl-tRNA synthetases from baker's yeast. Substrate specificity with regard to ATP analogs and mechanism of the aminoacylation reaction.来自面包酵母的苯丙氨酰 - tRNA合成酶和丝氨酰 - tRNA合成酶。关于ATP类似物的底物特异性及氨酰化反应机制。
Eur J Biochem. 1976 May 1;64(2):389-93. doi: 10.1111/j.1432-1033.1976.tb10313.x.
10
Steady state kinetic studies of initiation of RNA synthesis on T7 DNA in the presence of rifampicin.在利福平存在的情况下，对T7 DNA上RNA合成起始的稳态动力学研究。
Nucleic Acids Res. 1977 Nov;4(11):3863-76. doi: 10.1093/nar/4.11.3863.

ATP类似物在大肠杆菌RNA聚合酶催化的RNA合成起始过程中的特性。

The properties of ATP-analogs in initiation of RNA synthesis catalyzed by RNA polymerase from E coli.

作者信息

Smagowicz W J, Scheit K H

出版信息

Nucleic Acids Res. 1981 May 25;9(10):2397-410. doi: 10.1093/nar/9.10.2397.

DOI:10.1093/nar/9.10.2397
PMID:7019855
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC326853/
Abstract

Various base and sugar modified derivatives of ATP and UTP were used as substrate analogs for the steady state initiation reaction ATP+UTP=pppApU and the single step addition reaction ApC+ATP=ApCpA. These reactions were carried out by E. coli RNA polymerase on T7 DNA in the presence of rifampicin. The steady state kinetic parameters of the analogs, either as substrates or inhibitors, were determined. On the basis of the obtained results it is concluded that purine NTP s in initiation require anti-conformation about the glycosidic bonds as well as gauche-gauche conformation of the C(4')-C(5') bonds. The latter conformation is also a prerequisite for substrates in elongation, whereas strict anti-conformation of glycosidic bonds is not.

摘要

ATP和UTP的各种碱基和糖修饰衍生物被用作底物类似物，用于稳态起始反应ATP+UTP=pppApU和单步加成反应ApC+ATP=ApCpA。这些反应由大肠杆菌RNA聚合酶在利福平存在下于T7 DNA上进行。测定了类似物作为底物或抑制剂的稳态动力学参数。根据所得结果得出结论，起始过程中的嘌呤NTP需要糖苷键周围的反式构象以及C(4')-C(5')键的gauche-gauche构象。后一种构象也是延伸过程中底物的先决条件，而糖苷键的严格反式构象则不是。