Ohtani K, Okai K, Yamashita U, Yuasa I, Misaki A
Department of Food and Nutrition, Faculty of Human Life Science, Osaka City, Japan.
Biosci Biotechnol Biochem. 1995 Mar;59(3):378-81. doi: 10.1271/bbb.59.378.
An acidic polysaccharide was isolated from the water-soluble mucilage extracted from dried leaves of Corchorus olitorius, known as Moroheiya in Japan (3.0 g per 100 g). This polysaccharide showed a single peak in a Sepharose CL-6B column, and the specific rotation in H2O at 25 degrees C was +250 degrees. The polysaccharide was rich in uronic acid (65%), and consisted of rhamnose, glucose, galacturonic acid, and glucuronic acid in a molar ratio of 1.0:0.2:0.2:0.9:1.7, in addition to 3.7% of the acetyl group. A methylation analysis, Smith degradation study and fragmentation analysis suggested that this polysaccharide mainly consisted of O-4 substituted galacturonic acid and glucuronic acid, and O-2 substituted rhamnose residues, and that most of the (1-->4)-linked uronic acid residues were substituted at the O-3 position with glucuronic acid residues. This polysaccharide showed proliferative activity toward the murine splenocyte.
从黄麻叶（在日本称为Moroheiya）干燥叶中提取的水溶性黏液中分离出一种酸性多糖（每100克含3.0克）。这种多糖在Sepharose CL - 6B柱上呈现单峰，25℃水中的比旋度为+250°。该多糖富含糖醛酸（65%），除3.7%的乙酰基外，由鼠李糖、葡萄糖、半乳糖醛酸和葡萄糖醛酸以1.0:0.2:0.2:0.9:1.7的摩尔比组成。甲基化分析、史密斯降解研究和片段分析表明，这种多糖主要由O - 4取代的半乳糖醛酸和葡萄糖醛酸以及O - 2取代的鼠李糖残基组成，并且大多数(1→4)连接的糖醛酸残基在O - 3位被葡萄糖醛酸残基取代。这种多糖对小鼠脾细胞具有增殖活性。
