Hacker-Klom Ursula Beate
Klinik und Poliklinik für Strahlentherapie - Radioonkologie -, Universität, Albert-Schweitzer-Straße 33, D-48129 Münster, Bundesrepublik Deutschland.
Z Naturforsch C J Biosci. 1995 Mar-Apr;50(3-4):303-310. doi: 10.1515/znc-1995-3-421.
The dependence of spermatogenesis function on murine age shall be investigated. Thus, testicular samples of at least 10 NMRI mice per group aged 0 to 26 months are analysed by flow cytometry after staining the DNA with DAPI. The aim of this study is to be able to account for the influence of age on mice. There are no changes in spermatogenesis in mice aged 11 weeks up to 16 months with respect to testis weight and to the frequency of different testicular cell types. From 16 months onwards, there is a tendency to a reduced spermatogenesis function: The frequency of round spermatids is decreased. In addition, there is an increased chromatin dispersion in elongated spermatids with age. Thus, older mice (> 16 months) should not be used for experiments e.g. on radiation and drug effects any more. The frequency of abnormal diploid spermatozoa does not increase with age.
将研究精子发生功能对小鼠年龄的依赖性。因此，每组至少10只0至26个月大的NMRI小鼠的睾丸样本，在用DAPI对DNA染色后，通过流式细胞术进行分析。本研究的目的是能够解释年龄对小鼠的影响。在11周龄至16个月龄的小鼠中，就睾丸重量和不同睾丸细胞类型的频率而言，精子发生没有变化。从16个月起，精子发生功能有降低的趋势：圆形精子细胞的频率降低。此外，随着年龄的增长，伸长的精子细胞中染色质分散增加。因此，不再应将年龄较大的小鼠（>16个月）用于例如辐射和药物作用的实验。异常二倍体精子的频率不会随年龄增加。
