Hacker-Klom U B
Klinik und Poliklinik für Strahlentherapie-Radioonkologie-, Universität, Münster, Bundesrepublik Deutschland.
Z Naturforsch C J Biosci. 1994 Jul-Aug;49(7-8):522-5. doi: 10.1515/znc-1994-7-820.
We analysed testicular samples of NMRI mice every 2 h of a day in order to determine whether there is a circadian rhythm in spermatogenetic activity. We used flow cytometry after staining the DNA with DAPI. The highest proportion of DNA synthesizing cells (mainly spermatogonia and preleptotene spermatocytes) was seen at 8 p.m. and especially at 10 p.m.; the lowest proportion was observed at 2 p.m. At 6 a.m., the percentage of round spermatids increased significantly, whereas the fraction of 4c-cells decreased at that time. Our results of a diurnal rhythm of spermatogenic DNA synthesis rate are in contrast to another publication of other authors (Oakberg and Crosswait, 1983).
为了确定精子发生活动是否存在昼夜节律，我们每天每隔2小时分析一次NMRI小鼠的睾丸样本。在用DAPI对DNA进行染色后，我们使用了流式细胞术。DNA合成细胞（主要是精原细胞和前细线期精母细胞）比例最高的时候是晚上8点，尤其是晚上10点；比例最低的时候是下午2点。早上6点，圆形精子细胞的百分比显著增加，而此时4c细胞的比例下降。我们关于精子发生DNA合成速率昼夜节律的结果与其他作者的另一篇出版物（Oakberg和Crosswait，1983年）相反。
