Huang W, Akesson B, Svensson B G, Schütz A, Burk R F, Skerfving S
Department of Applied Nutrition and Food Chemistry, University of Lund, Sweden.
Br J Nutr. 1995 Mar;73(3):455-61. doi: 10.1079/bjn19950047.
In Sweden fish is considered to be an important source of dietary Se. Therefore Se status was assessed in forty-one middle-aged men with widely varying fish consumption. Glutathione peroxidase (EC 1.11.1.9) and selenoprotein P in plasma were measured by radioimmunoassay. Plasma Se among the men increased slightly with increasing consumption of fish, but no such increases in the concentrations of glutathione peroxidase and selenoprotein P in plasma were observed. Moreover, no correlation was found between plasma Se and glutathione peroxidase or selenoprotein P. Instead, glutathione peroxidase was significantly correlated with selenoprotein P (r 0.73, P < 0.001), indicating that both glutathione peroxidase and selenoprotein P were functional indicators of Se status in this group. The proportion of plasma Se located in glutathione peroxidase decreased with increasing plasma Se. The results suggest that the Se consumed from fish had no apparent effect on the amount of Se incorporated into the functional selenoproteins of plasma. It is concluded that in some cases selenoproteins may be better biological markers of Se status than the total concentration of Se.
在瑞典，鱼类被视为膳食中硒的重要来源。因此，对41名鱼类摄入量差异很大的中年男性的硒状况进行了评估。采用放射免疫分析法测定血浆中的谷胱甘肽过氧化物酶（EC 1.11.1.9）和硒蛋白P。这些男性的血浆硒水平随鱼类摄入量的增加而略有升高，但未观察到血浆中谷胱甘肽过氧化物酶和硒蛋白P浓度有类似增加。此外，血浆硒与谷胱甘肽过氧化物酶或硒蛋白P之间未发现相关性。相反，谷胱甘肽过氧化物酶与硒蛋白P显著相关（r = 0.73，P < 0.001），表明谷胱甘肽过氧化物酶和硒蛋白P都是该组中硒状况的功能指标。随着血浆硒水平的升高，位于谷胱甘肽过氧化物酶中的血浆硒比例下降。结果表明，从鱼类摄入的硒对血浆功能性硒蛋白中硒的掺入量没有明显影响。得出的结论是，在某些情况下，硒蛋白可能比硒的总浓度更能作为硒状况的良好生物学标志物。
