从斑叶疆南星块茎中纯化和鉴定一种N-乙酰乳糖胺特异性凝集素。

Purification and characterization of an N-acetyllactosamine-specific lectin from tubers of Arum maculatum.

作者信息

Allen A K

机构信息

Department of Biochemistry, Charing Cross and Westminster Medical School, London, UK.

出版信息

Biochim Biophys Acta. 1995 May 11;1244(1):129-32. doi: 10.1016/0304-4165(94)00210-o.

DOI:10.1016/0304-4165(94)00210-o

PMID:7766648

Abstract

A lectin was purified from the tubers of Arum maculatum (family Araceae) by affinity chromatography on a thyroglobulin-Sepharose column. The lectin is not a glycoprotein and has a subunit molecular weight of 14,600. It is specifically inhibited by N-acetyllactosamine (Gal beta 1,4GlcNAc), but is not significantly inhibited by monosaccharides or by lactose (Gal beta 1,4Glc), lacto-N-biose 1 (Gal beta 1,3GlcNAc), or chitobiose (GlcNAc beta 1,4GlcNAc). Asialoglycoproteins which contain N-acetyllactosamine structures are even more effective inhibitors of the lectin. This lectin should be a useful probe for N-acetyllactosamine groups in glycoproteins.

摘要

通过在甲状腺球蛋白-琼脂糖柱上进行亲和层析，从斑叶疆南星（天南星科）块茎中纯化出一种凝集素。该凝集素不是糖蛋白，亚基分子量为14,600。它被N-乙酰乳糖胺（Galβ1,4GlcNAc）特异性抑制，但不被单糖、乳糖（Galβ1,4Glc）、乳糖-N-二糖1（Galβ1,3GlcNAc）或壳二糖（GlcNAcβ1,4GlcNAc）显著抑制。含有N-乙酰乳糖胺结构的去唾液酸糖蛋白是该凝集素更有效的抑制剂。这种凝集素应该是用于检测糖蛋白中N-乙酰乳糖胺基团的有用探针。