Pribush A, Meyerstein D, Meyerstein N
Chemistry Department, Faculty of Natural Sciences, Ben-Gurion University of the Negev, Beer-Sheva, Israel.
Biochim Biophys Acta. 1995 May 17;1256(2):194-200. doi: 10.1016/0005-2760(95)00022-5.
Erythrocyte deformability was studied by continuous reading of sediment conductance during centrifugation. The decrease in sediment conductivity during centrifugation reflects erythrocyte deformation in the pellet. The degree of erythrocyte deformation depends on the duration of centrifugation and the magnitude of centripetal acceleration. When constant centrifugal force is applied over an extended period of time, a gradual decrease in pellet conductivity occurs. Stepwise enhancement of centripetal acceleration during centrifugation induces a rapid increase in erythrocyte deformation. After centrifugation, the relaxation of erythrocyte deformation is observed. However, the relaxation and the recovery of cell shape are incomplete. The difference in compressibility of previously centrifuged and noncentrifuged cells demonstrates that centrifugation causes irreversible alteration in erythrocyte deformability. The results show that the time-dependent resistance of erythrocyte sediment during centrifugation may serve as a useful index for the kinetics of erythrocyte deformation.
通过在离心过程中持续读取沉降电导来研究红细胞变形性。离心过程中沉降电导率的降低反映了沉淀中红细胞的变形情况。红细胞变形程度取决于离心时间和向心加速度的大小。当在较长时间内施加恒定离心力时，沉淀电导率会逐渐降低。离心过程中向心加速度的逐步增强会导致红细胞变形迅速增加。离心后，可观察到红细胞变形的松弛现象。然而，细胞形状的松弛和恢复并不完全。先前离心和未离心细胞在压缩性上的差异表明，离心会导致红细胞变形性发生不可逆改变。结果表明，离心过程中红细胞沉降的时间依赖性阻力可作为红细胞变形动力学的一个有用指标。
