Pribush A, Meyerstein D, Meyerstein N
Chemistry Department, Faculty of Natural Sciences, Ben-Gurion University of the Negev, Beer-Sheva, Israel.
Biochim Biophys Acta. 1995 May 17;1256(2):187-93. doi: 10.1016/0005-2760(95)00021-4.
Sedimentation of hardened erythrocytes in a centrifugal field was studied by time recording of the current chamber in the longitudinal and the transversal directions relative to the cells' movement. The results clearly indicate the existence of an erythrocyte concentration profile during centrifugation. The rates of both longitudinal and transversal current alteration increase with centripetal acceleration and with falling cell concentration. The pellet formed from hardened cells represents virtually incompressible body. It is shown that erythrocyte shape affects the pellet conductivity. Analysis of the data using the modified Stokes' law enables calculation of the cell size distribution. The modal size of macrocytes, normal erythrocytes and two samples of microcytes thus measured was 3.40, 3.01, 2.63 and 2.83 microns, respectively. These data demonstrate that conductometric analysis is useful for investigating abnormalities in erythrocyte size.
通过记录电流室相对于细胞运动的纵向和横向方向上的时间，研究了硬化红细胞在离心场中的沉降。结果清楚地表明在离心过程中存在红细胞浓度分布。纵向和横向电流变化率均随向心加速度和细胞浓度降低而增加。由硬化细胞形成的沉淀几乎代表不可压缩体。结果表明红细胞形状会影响沉淀的电导率。使用修正的斯托克斯定律对数据进行分析能够计算细胞大小分布。如此测量的大红细胞、正常红细胞以及两个小红细胞样本的众数大小分别为3.40、3.01、2.63和2.83微米。这些数据表明电导分析对于研究红细胞大小异常是有用的。
