对来自酵母的tRNAPhe氨酰化过程中活性中间体的一项测试。 - Suppr | 超能文献

A test for the active intermediate in the aminoacylation of tRNAPhe from yeast.

作者信息

Thiebe R, Hirsch R

出版信息

FEBS Lett. 1975 Dec 15;60(2):338-41. doi: 10.1016/0014-5793(75)80744-7.

DOI:10.1016/0014-5793(75)80744-7

Abstract

摘要

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A test for the active intermediate in the aminoacylation of tRNAPhe from yeast.对来自酵母的tRNAPhe氨酰化过程中活性中间体的一项测试。

FEBS Lett. 1975 Dec 15;60(2):338-41. doi: 10.1016/0014-5793(75)80744-7.

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A new active intermediate in the aminoacylation of tRNA.一种参与tRNA氨酰化反应的新型活性中间体。

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Catalytical mechanism of the phenylalanyl-tRNA synthetase from yeast. Reactivity of ATP in the absence of phenylalanine.酵母苯丙氨酰 - tRNA合成酶的催化机制。无苯丙氨酸时ATP的反应活性。

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Fast kinetic study of yeast phenylalanyl-tRNA synthetase: an efficient discrimination between tyrosine and phenylalanine at the level of the aminoacyladenylate-enzyme complex.酵母苯丙氨酰 - tRNA合成酶的快速动力学研究：在氨酰腺苷酸 - 酶复合物水平上对酪氨酸和苯丙氨酸的有效区分。

Biochemistry. 1983 Feb 1;22(3):681-9. doi: 10.1021/bi00272a024.

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Yeast phenylalanyl-tRNA synthetase: evidence for the triggering of an AMP--ATP exchange by tRNA.酵母苯丙氨酰 - tRNA合成酶：tRNA触发AMP - ATP交换的证据。

FEBS Lett. 1976 Jan 1;61(1):28-31. doi: 10.1016/0014-5793(76)80163-9.

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Conformational activation of the yeast phenylalanyl-tRNA synthetase catalytic site induced by tRNAPhe interaction: triggering of adenosine or CpCpA trinucleoside diphosphate aminoacylation upon binding of tRNAPhe lacking these residues.由tRNAPhe相互作用诱导的酵母苯丙氨酰-tRNA合成酶催化位点的构象激活：缺乏这些残基的tRNAPhe结合时腺苷或CpCpA三核苷二磷酸氨基酰化的触发。

Proc Natl Acad Sci U S A. 1981 Mar;78(3):1606-8. doi: 10.1073/pnas.78.3.1606.

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Yeast phenylalanyl--tRNA synthetase. Stimulation of the hydrolysis of enzyme bound aminoacyladenylate upon binding of tRNA.酵母苯丙氨酰 - tRNA合成酶。tRNA结合后对酶结合的氨酰腺苷酸水解的刺激作用。

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Yeast phenylalanyl-tRNA synthetase: evidence for the formation of ADP by phosphorolysis of enzyme-bound aminoacyladenylate.酵母苯丙氨酰 - tRNA合成酶：通过酶结合的氨酰腺苷酸的磷酸解作用形成ADP的证据。

Biochimie. 1987 Nov-Dec;69(11-12):1175-81. doi: 10.1016/0300-9084(87)90144-1.

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On the stereochemistry of activation of phenylalanine by phenylalanyl-tRNA synthetase from baker's yeast.关于面包酵母苯丙氨酰 - tRNA合成酶激活苯丙氨酸的立体化学研究。

Eur J Biochem. 1977 Jun 1;76(1):263-7. doi: 10.1111/j.1432-1033.1977.tb11591.x.

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Lack of correlation between affinity of the tRNA for the aminoacyl-tRNA synthetase and aminoacylation capacity as studied with modified tRNAPhe.用修饰的苯丙氨酸转运RNA（tRNAPhe）研究发现，tRNA对氨酰-tRNA合成酶的亲和力与氨酰化能力之间缺乏相关性。

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引用本文的文献

1

Serine activation is the rate limiting step of tRNASer aminoacylation by yeast seryl tRNA synthetase.丝氨酸激活是酵母丝氨酰-tRNA合成酶催化tRNASer氨酰化的限速步骤。

Nucleic Acids Res. 1980 Sep 11;8(17):4021-39. doi: 10.1093/nar/8.17.4021.

2

Aminoacyl-tRNA synthetases catalyze AMP----ADP----ATP exchange reactions, indicating labile covalent enzyme-amino-acid intermediates.氨酰-tRNA合成酶催化AMP----ADP----ATP交换反应，表明存在不稳定的共价酶-氨基酸中间体。

Proc Natl Acad Sci U S A. 1987 Nov;84(22):7891-5. doi: 10.1073/pnas.84.22.7891.