Ishimaru H, Takahashi A, Ikarashi Y, Maruyama Y
Department of Neuropsychopharmacology (Tsumura), Gunma University School of Medicine, Japan.
Neuroreport. 1995 Feb 15;6(3):557-60. doi: 10.1097/00001756-199502000-00037.
We investigated alterations in cholinergic neurones in the gerbil hippocampus after ischaemia. The cholinergic function of acetylcholine (ACh) release fluctuated over the test period. Choline acetyltransferase (ChAT) immunoreactivity decreased slightly on day 1 and no ChAT immunoreactivity was observed on or after day 4 after ischaemia. Since ChAT immunoreactivity is a marker of cholinergic terminal survival, post-ischaemic cholinergic dysfunction on and after day 4 was accompanied by the destruction of terminals. However, dysfunction of the cholinergic system without destruction of the terminals is possible since mild ischaemia decreases ACh release in spite of retaining intact ChAT immunoreactivity. In the morphological study, delayed neuronal death in the stratum pyramidale was observed from day 4. The present study shows that presynaptic cholinergic dysfunction occurs in the early stage prior to pyramidal cell death.
我们研究了沙土鼠海马在缺血后胆碱能神经元的变化。在测试期间，乙酰胆碱（ACh）释放的胆碱能功能波动。胆碱乙酰转移酶（ChAT）免疫反应性在缺血后第1天略有下降，在第4天及之后未观察到ChAT免疫反应性。由于ChAT免疫反应性是胆碱能终末存活的标志物，缺血后第4天及之后的缺血后胆碱能功能障碍伴随着终末的破坏。然而，胆碱能系统功能障碍而终末未被破坏是可能的，因为轻度缺血尽管保留完整的ChAT免疫反应性，但会降低ACh释放。在形态学研究中，从第4天开始观察到锥体层延迟性神经元死亡。本研究表明，突触前胆碱能功能障碍发生在锥体细胞死亡之前的早期阶段。
