Kopriva S, Turner S R, Rawsthorne S, Bauwe H
Department of Molecular Cell Biology, Institute of Plant Genetics and Crop Plant Research, Gatersleben, Germany.
Plant Mol Biol. 1995 Mar;27(6):1215-20. doi: 10.1007/BF00020895.
We have isolated and sequenced cDNA clones encoding T-protein of the glycine decarboxylase complex from three plant species, Flaveria pringlei, Solanum tuberosum and Pisum sativum. The predicted amino acid sequences of these clones are at least 87% identical and all are similar to the predicted sequences of the bovine, human, chicken and Escherichia coli T-proteins. Alignment of all these sequences revealed conserved domains, one of which showed a significant similarity to a part of the formyltetrahydrofolate synthetases from procaryotes and eucaryotes. This suggests that the T-protein sequence is not as unique as previously thought.
我们已经从三种植物——普氏黄缨菊、马铃薯和豌豆中分离并测序了编码甘氨酸脱羧酶复合体T蛋白的cDNA克隆。这些克隆预测的氨基酸序列至少87%相同，并且都与牛、人、鸡和大肠杆菌T蛋白的预测序列相似。所有这些序列的比对揭示了保守结构域，其中一个结构域与原核生物和真核生物的甲酰四氢叶酸合成酶的一部分显示出显著的相似性。这表明T蛋白序列并不像之前认为的那样独特。
