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从大豆(Glycine max)中分离并鉴定出gmsti,这是一个应激诱导基因,编码一种属于TPR(四肽重复序列)家族的蛋白质。

Isolation and characterization of gmsti, a stress-inducible gene from soybean (Glycine max) coding for a protein belonging to the TPR (tetratricopeptide repeats) family.

作者信息

Hernández Torres J, Chatellard P, Stutz E

机构信息

Laboratoire de Biochimie végétale, Université de Neuchâtel, Switzerland.

出版信息

Plant Mol Biol. 1995 Mar;27(6):1221-6. doi: 10.1007/BF00020896.

DOI:10.1007/BF00020896
PMID:7766904
Abstract

In close vicinity of two fus nuclear genes (chloroplast-specific translation elongation factor cEF-G) of soybean (Glycine max) we localized a split nuclear gene coding for a protein with tetratricopeptide repeats (TPR). A full-length cDNA was sequenced (1871 nucleotides). It encodes a protein (569 amino acids) with high sequence identity to the yeast STI1 stress-inducible and the human transformation-sensitive IEF SSP 3521 protein which both carry TPR elements. The soybean gene is heat-inducible. This is the first evidence for the existence of plant genes coding for proteins which belong to the TPR family. We call the gene gmsti and the protein GMSTI in analogy to the yeast counterpart.

摘要

在大豆(Glycine max)的两个叶绿体核基因(叶绿体特异性翻译延伸因子cEF - G)附近,我们定位了一个编码具有四肽重复序列(TPR)蛋白的断裂核基因。对一个全长cDNA进行了测序(1871个核苷酸)。它编码一种蛋白质(569个氨基酸),与酵母STI1应激诱导蛋白和人类转化敏感IEF SSP 3521蛋白具有高度序列同一性,这两种蛋白都带有TPR元件。大豆基因是热诱导型的。这是首次证明存在编码属于TPR家族蛋白质的植物基因。我们将该基因命名为gmsti,将该蛋白命名为GMSTI,以类比酵母中的对应物。

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