Böhm F, Dvorák R
Folia Biol (Praha). 1976;22(2):87-9.
Mouse plaque-forming cells were kept at--196 degrees C for different periods of time up to 37 weeks. The decrease in cell number occurring during the storage was found to be in no correlation with the length of the storage time. The cryoprotective effectiveness of dimethyl sulphoxide upon survival of cells was equal for concentrations ranging from 5-15%.
将小鼠空斑形成细胞在-196℃下保存不同时长,最长达37周。结果发现,保存期间细胞数量的减少与保存时间长短无关。二甲基亚砜在5%-15%的浓度范围内,对细胞存活的冷冻保护效果相同。