利用一小群体细胞杂种对人类11号染色体q臂上已有的和新的标记进行定位及精细图谱绘制。
Placement and refined mapping of established and new markers on human chromosome 11q using a small panel of somatic cell hybrids.
作者信息
Evans K L, van Heyningen V, Porteous D J
机构信息
MRC Human Genetics Unit, Western General Hospital, Edinburgh, UK.
出版信息
Eur J Hum Genet. 1995;3(1):42-8. doi: 10.1159/000472272.
Constructing detailed chromosome maps relies on the ability to place new markers rapidly and accurately. Here we map two new anonymous DNA markers precisely on chromosome 11q and refine the location of established markers using a panel of just six fragmentation hybrids, with reference to four translocation chromosomes. As several of the hybrids retain multiple segments of chromosome 11, it is possible to quickly assign new markers to subintervals using this modest set of hybrid DNAs.
构建详细的染色体图谱依赖于快速且准确地定位新标记的能力。在此，我们将两个新的匿名DNA标记精确地定位在11号染色体长臂上，并借助仅由六个片段化杂种组成的一组样本，参照四条易位染色体，对已确定标记的位置进行了优化。由于其中几个杂种保留了11号染色体的多个片段，因此利用这组数量不多的杂种DNA能够迅速将新标记分配到各个亚区间。
Zotero 插件