人类13号染色体的体细胞杂种图谱。

A somatic cell hybrid map of human chromosome 13.

作者信息

Washington S S, Bowcock A M, Gerken S, Matsunami N, Lesh D, Osborne-Lawrence S L, Cowell J, Ledbetter D H, White R L, Chakravarti A

机构信息

Department of Human Genetics, University of Pittsburgh, Pennsylvania 15261.

出版信息

Genomics. 1993 Dec;18(3):486-95. doi: 10.1016/s0888-7543(11)80004-2.

DOI:10.1016/s0888-7543(11)80004-2

PMID:8307557

Abstract

We have constructed a chromosome 13 somatic cell hybrid map using seven cell lines: PGMEA6, a hybrid containing the entire chromosome 13, and six hybrids containing various deletions of chromosome 13 (BARF7, PPF22, KBF11, KSF39, CF25, and CF27). We have mapped 80 markers that define 10 regions of chromosome 13 with respect to 10 breakpoints in the mapping panel; these regions range in size from 4 to 24 Mb, with an average size of 8 Mb. The 80 markers sublocalized on our mapping panel include 10 Alu-PCR clones, 6 of which were converted to sequence-tagged sites; 40 (CA)n repeat-containing clones, 27 of which are microsatellite PCR markers; 8 (AAAG)n repeat-containing PCR markers, 1 two-allele PCR marker, 4 genes or expressed sequences, and 17 anonymous DNA probes. This low-resolution physical map can be used as a backbone map for more refined physical mapping using radiation hybrids or yeast artificial chromosomes.

摘要

我们利用七个细胞系构建了13号染色体体细胞杂交图谱：PGMEA6，一个包含完整13号染色体的杂种细胞系，以及六个包含13号染色体各种缺失片段的杂种细胞系（BARF7、PPF22、KBF11、KSF39、CF25和CF27）。我们已对80个标记进行了定位，这些标记相对于定位板中的10个断点确定了13号染色体的10个区域；这些区域大小从4兆碱基到24兆碱基不等，平均大小为8兆碱基。在我们的定位板上进行亚定位的80个标记包括10个Alu-PCR克隆，其中6个被转化为序列标签位点；40个含（CA）n重复序列的克隆，其中27个是微卫星PCR标记；8个含（AAAG）n重复序列的PCR标记，1个双等位基因PCR标记，4个基因或表达序列，以及17个匿名DNA探针。这个低分辨率的物理图谱可作为使用辐射杂种细胞或酵母人工染色体进行更精细物理图谱构建的骨干图谱。