[肝细胞癌患者丙型肝炎病毒的基因分型]

[Genotyping of hepatitis C virus in patients with hepatocellular carcinoma].

作者信息

Cui X H, Pan W, Qi Z T

机构信息

Department of Microbiology, Second Military Medical University, Shanghai.

出版信息

Zhonghua Yi Xue Za Zhi. 1995 Feb;75(2):92-3, 126-7.

PMID:7767776

Abstract

Forty-two patients with hepatocellular carcinoma (HCC) were examined for hepatitis C virus (HCV) RNA in liver tissues by reverse transcription-polymerase chain reaction (RT PCR). Typing of HCV liver samples of 18 patients was dependent on the amplification of NS5 region by PCR using type-specific primers. Type-II was found in 14 of the 18 patients (78%), 7 of the 18 patients (39%) and 4 of the 18 patients (22%) were positive for type-II and I and for type-II and III or IV (III/IV), respectively. Type V or VI (V/VI) infection was not observed. These data indicate that HCV type-II may be the major type in HCC patients with HCV infection in China, and some patients can be coinfected with type-II and I or III/IV.

摘要

采用逆转录聚合酶链反应（RT PCR）对42例肝细胞癌（HCC）患者的肝组织进行丙型肝炎病毒（HCV）RNA检测。18例患者的HCV肝样本分型取决于使用型特异性引物通过PCR扩增NS5区域。18例患者中有14例（78%）检测到Ⅱ型，18例患者中有7例（39%）Ⅱ型和Ⅰ型呈阳性，18例患者中有4例（22%）Ⅱ型和Ⅲ型或Ⅳ型（Ⅲ/Ⅳ型）呈阳性。未观察到Ⅴ型或Ⅵ型（Ⅴ/Ⅵ型）感染。这些数据表明，在中国，HCVⅡ型可能是HCV感染的HCC患者中的主要类型，一些患者可能同时感染Ⅱ型和Ⅰ型或Ⅲ/Ⅳ型。