[中国肝癌患者肿瘤组织中的丙型肝炎病毒RNA]

[Hepatitis C virus RNA in tumor tissues of Chinese liver cancer patients].

作者信息

Zhang C X, Lu Y P, Yu Z Y

机构信息

Department of Molecular Virology, Shanghai Medical University.

出版信息

Zhonghua Yi Xue Za Zhi. 1994 Nov;74(11):673-5, 709.

PMID:7866902

Abstract

A total of 38 specimens of liver cancer and pericancerous tissues from 30 hepatocellular carcinoma patients were studied for hepatitis C virus (HCV) RNA by RT-PCR using primers from the s'non-coding regions of HCV viral genome. Serum anti-HCV of these patients was also assayed as a marker of infection. The results showed that 10/30 (33.3%) hepatocellular carcinoma (HCC) patients were infected by HCV, while 23/30 (76.7%) by hepatitis B virus (HBV). The HCV RNA from liver cancer tissues were characterized by type II and III through restriction enzyme digestion. Sequencing of the 5'-NC regions amplified revealed that this region was conserved. Since 8 of the 10 HCV infected HCC patients were coinfected with HBV, chronic infection caused by HCV and HBV might be one of the factors leading to HCC.

摘要

使用来自丙型肝炎病毒（HCV）病毒基因组5'非编码区的引物，通过逆转录聚合酶链反应（RT-PCR）对30例肝细胞癌患者的38份肝癌组织和癌旁组织标本进行丙型肝炎病毒（HCV）RNA研究。还检测了这些患者的血清抗-HCV作为感染标志物。结果显示，10/30（33.3%）的肝细胞癌（HCC）患者感染了HCV，而23/30（76.7%）的患者感染了乙型肝炎病毒（HBV）。通过限制性内切酶消化，肝癌组织中的HCV RNA被鉴定为II型和III型。对扩增的5'-NC区域进行测序显示该区域是保守的。由于10例感染HCV的HCC患者中有8例同时感染了HBV，HCV和HBV引起的慢性感染可能是导致HCC的因素之一。