Zhang J Z, Fan M Y, Bi D Z
Department of Rickettsiology, Chinese Academy of Preventive Medicine, Beijing.
Zhonghua Liu Xing Bing Xue Za Zhi. 1995 Feb;16(1):25-8.
It was the first time that a primer pairs derived from the 190KDa protein antigen gene of R. rickettsii were used to amplify SFGR DNA in ticks, tick ova, larva, tick faeces and rodent organs which were collected in Hebei, Heilongjiang, Hainan and Beijing. A 532bp fragment was respectively amplified from above samples. The results were partially in concordance with data obtained through rickettsiae isolation. It was suggested that PCR is a rapid, specific, sensitive and practical method for detection of SFGR in endemic foci.
这是首次使用源自立氏立克次体190KDa蛋白抗原基因的引物对，对采自河北、黑龙江、海南和北京的蜱虫、蜱卵、幼虫、蜱粪便及啮齿动物器官中的斑点热群立克次体（SFGR）DNA进行扩增。从上述样本中分别扩增出一个532bp的片段。结果与通过立克次体分离获得的数据部分一致。提示PCR是一种用于在疫源地检测SFGR的快速、特异、灵敏且实用的方法。
