采用聚合酶链反应/限制性片段长度多态性技术检测福建省宁化县蜱类和鼠类中的斑点热群立克次体。
Using PCR/RFLP to detect spotted fever group rickettsia in ticks and rodents collected in Ninghua, Fujian province.
作者信息
Chen Zhenguang, Chen Min, Zhong Jianping, Bi Dezeng
机构信息
Ninghua County Anti-Epidemic Station, Fujian 365400, China.
出版信息
Zhonghua Yu Fang Yi Xue Za Zhi. 2002 Mar;36(2):106-8.
OBJECTIVE
To study the existence of natural foci of Spotted Fever in Ninghua, Fujian province.
METHODS
Using DNA polymerase chain reaction and restriction endonuclease fragment length polymorphism analysis (PCR/RFLP) to detect spotted fever group rickettsiae (SFGR) in ticks and rodents.
RESULTS
It was found that H. wellingtoni, H. yeni, and Dermacentor auratus were infected with Rickettsia sibirica; the DNA fragments were cloned, the PCR products from isolated strain NH-97 were antigenically and genotypically identical to Rickettsia sibirica. Rattus flavipectus were found infected with R. conorii. One of the sequeuce analysis showed that the DNA sequence was different from other SFGR and close to R. japanic.
CONCLUSION
Natural foci of R. sibirica, R. sibrica, R. japanic and R.conorii are found in Ninghua, Fujian province of China.
目的
研究福建省宁化县斑点热自然疫源地的存在情况。
方法
采用DNA聚合酶链反应和限制性内切酶片段长度多态性分析（PCR/RFLP）检测蜱和啮齿动物中的斑点热群立克次体（SFGR）。
结果
发现威氏硬蜱、叶氏硬蜱和金黄革蜱感染了西伯利亚立克次体；对DNA片段进行了克隆，分离菌株NH-97的PCR产物在抗原性和基因型上与西伯利亚立克次体相同。发现黄胸鼠感染了康氏立克次体。序列分析之一表明，该DNA序列与其他SFGR不同，与日本立克次体相近。
结论
在中国福建省宁化县发现了西伯利亚立克次体、日本立克次体和康氏立克次体的自然疫源地。
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