Bånrud H, Stokke T, Moan J, Berg K
Department of Biophysics, Institute for Cancer Research, Montebello, Oslo, Norway.
Carcinogenesis. 1995 May;16(5):1087-94. doi: 10.1093/carcin/16.5.1087.
The effects of UVA (365 nm) and UVB (297/302 nm) radiation on cellular proliferation, cell cycle progression, aneuploidy and multinucleus induction have been studied in two different fibroblast cell lines; V79 Chinese hamster lung fibroblasts and 3T3 Swiss albino mouse fibroblasts. UVA and UVB were found to inhibit proliferation of the cells in a fluence-dependent manner. This inhibition was due to a temporary accumulation of cells in the S phase of the cell cycle, as determined by flow cytometry of UV-irradiated V79 cells. The UVA- and UVB-induced S phase delay was observed a few hours after irradiation and by 48 h post-irradiation the cells had recovered from cell cycle arrest. For UVA, but not for UVB, the elongation of S phase was followed by a small accumulation of cells in the G2 phase. After exposure to UVA and a post-irradiation time long enough for the cells to recover from cell cycle arrest, a large proportion of the cells were polyploid, with two or more nuclei. Multinucleated cells were not, however, induced by UVB irradiation.
在两种不同的成纤维细胞系中，即V79中国仓鼠肺成纤维细胞和3T3瑞士白化小鼠成纤维细胞，研究了UVA（365纳米）和UVB（297/302纳米）辐射对细胞增殖、细胞周期进程、非整倍体和多核诱导的影响。发现UVA和UVB以通量依赖的方式抑制细胞增殖。通过对紫外线照射的V79细胞进行流式细胞术测定，这种抑制是由于细胞在细胞周期的S期暂时积累所致。在照射后数小时观察到UVA和UVB诱导的S期延迟，到照射后48小时，细胞已从细胞周期停滞中恢复。对于UVA而非UVB，S期延长后G2期有少量细胞积累。在暴露于UVA且照射后时间足够长以使细胞从细胞周期停滞中恢复后，很大一部分细胞是多倍体，有两个或更多细胞核。然而，UVB照射并未诱导产生多核细胞。
