垂体腺苷酸环化酶激活肽对麻醉犬脑动脉及椎动脉血流的影响。

The effects of pituitary adenylate cyclase-activating polypeptide on cerebral arteries and vertebral artery blood flow in anesthetized dogs.

作者信息

Seki Y, Suzuki Y, Baskaya M K, Kano T, Saito K, Takayasu M, Shibuya M, Sugita K

机构信息

Department of Neurosurgery, Nagoya University School of Medicine, Japan.

出版信息

Eur J Pharmacol. 1995 Mar 14;275(3):259-66. doi: 10.1016/0014-2999(95)00011-9.

DOI:10.1016/0014-2999(95)00011-9

PMID:7768293

Abstract

We investigated and compared the effects of pituitary adenylate cyclase-activating polypeptide (PACAP) and vasoactive intestinal peptide (VIP) on cerebral circulation in anesthetized dogs. The intracisternal administration of PACAP-27, PACAP-38, and VIP dilated canine cerebral arteries in a dose-dependent manner. A 10 nmol dose of PACAP-27, PACAP-38, and VIP dilated the basilar artery by 23 +/- 3, 27 +/- 3 and 30 +/- 3%, respectively. Rostrally located arteries tended to be more responsive to PACAP-27. Pretreatment with NG-monomethyl-L-arginine did not affect PACAP-27-induced vasodilation. Vertebral artery blood flow was also affected by intra-arterial injection of these peptides in a dose-dependent manner. A 100 pmol dose of PACAP-27, PACAP-38, and VIP increased the vertebral artery blood flow by 42 +/- 10, 29 +/- 4, and 62 +/- 11%, respectively. The VIP receptor antagonist, [Lys1,Pro2,5,Arg3,4,Tyr6]VIP, inhibited both the VIP- and PACAP-38-induced increase in vertebral artery blood flow. These findings suggest that PACAP plays a role in the regulation of cerebral circulation.

摘要

我们研究并比较了垂体腺苷酸环化酶激活多肽（PACAP）和血管活性肠肽（VIP）对麻醉犬脑循环的影响。脑池内给予PACAP - 27、PACAP - 38和VIP可使犬脑动脉呈剂量依赖性扩张。10 nmol剂量的PACAP - 27、PACAP - 38和VIP分别使基底动脉扩张23±3%、27±3%和30±3%。位于头端的动脉对PACAP - 27的反应往往更强。用NG - 单甲基 - L - 精氨酸预处理不影响PACAP - 27诱导的血管舒张。椎动脉血流也受到这些肽动脉内注射的剂量依赖性影响。100 pmol剂量的PACAP - 27、PACAP - 38和VIP分别使椎动脉血流增加42±10%、29±4%和62±11%。VIP受体拮抗剂[Lys1,Pro2,5,Arg3,4,Tyr6]VIP可抑制VIP和PACAP - 38诱导的椎动脉血流增加。这些发现表明PACAP在脑循环调节中发挥作用。