基质金属蛋白酶-2和金属蛋白酶组织抑制剂-2在儿科肿瘤细胞中的表达。肿瘤细胞增殖调节剂对明胶酶活性的影响。
Matrix metalloproteinase-2 and tissue inhibitor of metalloproteinase-2 expression in paediatric tumour cells. Effects of tumour cell proliferation modulators on gelatinolytic activity.
作者信息
de Veas R G, Schweigerer L, Medina M A
机构信息
Laboratorio de Bioquímica y Biología Molecular, Facultad de Ciencias, Universidad de Málaga, Spain.
出版信息
J Cancer Res Clin Oncol. 1995;121(5):275-8. doi: 10.1007/BF01209593.
Abstract
We have examined the expression of 72-kDa gelatinase/type IV collagenase or matrix metalloproteinase-2 (MMP-2) and its inhibitor, tissue inhibitor of metalloproteinase-2 (TIMP-2), in various cell lines derived from paediatric tumours. In a neuroblastoma model system of tumour progression, the expression level of MMP-2 mRNA was higher in the more malignant cell line. Surprisingly, MMP-2 was not expressed in the highly malignant rhabdomyosarcoma A-204 cell line. TIMP-2 showed higher expression levels in the 007 and U-2OS tumour cell lines than in the more malignant ones, WAC2 and A-204 cells. We have also determined the effect of some tumour cell proliferation modulators on gelatinolytic activity. While basic fibroblast growth factor and retinoic acid produced no apparent change in gelatinolytic activity, genistein induced in partial inhibition of gelatinolytic activity.
摘要
我们检测了72-kDa明胶酶/IV型胶原酶或基质金属蛋白酶-2（MMP-2）及其抑制剂金属蛋白酶组织抑制剂-2（TIMP-2）在源自小儿肿瘤的各种细胞系中的表达。在肿瘤进展的神经母细胞瘤模型系统中，MMP-2 mRNA的表达水平在恶性程度更高的细胞系中更高。令人惊讶的是，MMP-2在高度恶性的横纹肌肉瘤A-204细胞系中未表达。TIMP-2在007和U-2OS肿瘤细胞系中的表达水平高于恶性程度更高的WAC2和A-204细胞。我们还确定了一些肿瘤细胞增殖调节剂对明胶酶活性的影响。虽然碱性成纤维细胞生长因子和视黄酸对明胶酶活性没有明显影响，但染料木黄酮可部分抑制明胶酶活性。
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