Hozák P, Sasseville A M, Raymond Y, Cook P R
CRC Nuclear Structure and Function Research Group, Sir William Dunn School of Pathology, University of Oxford, UK.
J Cell Sci. 1995 Feb;108 ( Pt 2):635-44. doi: 10.1242/jcs.108.2.635.
The nuclear lamina forms a protein mesh that underlies the nuclear membrane. In most mammalian cells it contains the intermediate filament proteins, lamins A, B and C. As their name indicates, lamins are generally thought to be confined to the nuclear periphery. We now show that they also form part of a diffuse skeleton that ramifies throughout the interior of the nucleus. Unlike their peripheral counterparts, these internal lamins are buried in dense chromatin and so are inaccessible to antibodies, but accessibility can be increased by removing chromatin. Knobs and nodes on an internal skeleton can then be immunolabelled using fluorescein- or gold-conjugated anti-lamin A antibodies. These results suggest that the lamins are misnamed as they are also found internally.
核纤层形成一个位于核膜下方的蛋白质网络。在大多数哺乳动物细胞中，它包含中间丝蛋白，核纤层蛋白A、B和C。顾名思义，核纤层蛋白通常被认为局限于核周边。我们现在表明，它们还构成了一个弥漫性骨架的一部分，该骨架在细胞核内部呈分支状分布。与核周边的核纤层蛋白不同，这些内部核纤层蛋白埋在致密染色质中，因此抗体无法接近，但通过去除染色质可以增加其可及性。然后可以使用荧光素或金偶联的抗核纤层蛋白A抗体对内部骨架上的瘤和节进行免疫标记。这些结果表明，核纤层蛋白被误称了，因为它们在细胞核内部也有发现。
