Manandhar G, Onishchenko G E
Department of Cytology and Histology, Biology Faculty, Moscow State University, Russia.
J Cell Sci. 1995 Feb;108 ( Pt 2):667-73. doi: 10.1242/jcs.108.2.667.
Ultrastructure of centrioles of fused cells containing heterophasic interphase nuclei, premature chromosome condensation, and a telophase-like nucleus were studied. The study indicated that the heterophasic cellular environments contributed by the different cell partners exert a mutually opposite effect on the structure of centrioles. The G1-cell partner suppresses replication of S-centrioles. Asynchronous replications of G1 and S-stage centrioles were observed in some G1-S, G1-G2 and S-G2 fused cells. In interphase-mitotic fused cells, centrioles of interphase stages underwent mitotic activation when their nuclei were induced to premature chromosome condensation. Daughter centrioles of G1-, S-, and G2-stages were also capable of mitotic activation independently if they were separated from their mother centriole. Inactive centrioles were observed in some cells containing G1-premature chromosome condensation. When mitotic nuclei were induced to telophase-like nucleus formation, their centrioles were also inactivated. Concomitant events of induced nuclear and centriolar changes suggest that they might have been controlled by the heterophasic cytoplasmic factors through similar pathways.
对含有异相间期核、早熟染色体凝集和末期样核的融合细胞的中心粒超微结构进行了研究。研究表明，不同细胞伙伴所贡献的异相细胞环境对中心粒结构产生相互相反的影响。G1期细胞伙伴抑制S期中心粒的复制。在一些G1-S、G1-G2和S-G2融合细胞中观察到G1期和S期中心粒的异步复制。在间期-有丝分裂融合细胞中，当间期阶段的中心粒的核被诱导早熟染色体凝集时，它们会经历有丝分裂激活。如果G1期、S期和G2期的子中心粒与它们的母中心粒分离，它们也能够独立地进行有丝分裂激活。在一些含有G1期早熟染色体凝集的细胞中观察到无活性的中心粒。当有丝分裂核被诱导形成末期样核时，它们的中心粒也会失活。诱导的核和中心粒变化的伴随事件表明，它们可能通过相似的途径受异相细胞质因子的控制。
