通过等温扩增法（核酸序列依赖性扩增）检测血浆中的HIV-1 RNA，与HIV-1感染阶段无关。

Detection of HIV-1 RNA in plasma by isothermal amplification (NASBA) irrespective of the stage of HIV-1 infection.

作者信息

Zaaijer H L, Kok W, ten Veen J H, Reesink H W, Foolen H, Winkel I N, Huisman J G, Cuypers H T, Kievits T, Lelie P N

机构信息

Central Laboratory of the Netherlands Red Cross Blood Transfusion Service (CLB), Amsterdam.

出版信息

J Virol Methods. 1995 Mar;52(1-2):175-81. doi: 10.1016/0166-0934(94)00160-i.

DOI:10.1016/0166-0934(94)00160-i

PMID:7769031

Abstract

Using an experimental assay for isothermal amplification of HIV RNA in plasma (NASBA, Organon Teknika, Boxtel, The Netherlands), 70 samples from 59 HIV-1-infected persons and 29 samples from 28 uninfected volunteer blood donors were tested for the presence of HIV-1 RNA. The HIV-1-positive plasma samples were drawn from patients at various stages of infection: two samples from persons with signs of acute HIV infection (CDC stage I); 29 samples from 25 symptom-free persons (CDC stage II) and 39 samples from 32 persons with AIDS-related symptoms (CDC stage IV). All samples from HIV-1-infected persons had HIV-1 RNA, irrespective of the stage of infection (100% sensitivity). Testing the donor samples in duplicate, initially 54/58 samples (93%) tested negative for HIV-1 RNA. Repeated testing of the 4 samples with inconsistent test results showed all samples to be negative (specificity 100%). Detection of HIV-1 RNA in plasma by isothermal amplification (NASBA) promises to be a valuable alternative to the detection of HIV-1 RNA or DNA by the polymerase chain reaction.

摘要

采用一种用于血浆中HIV RNA等温扩增的实验检测方法（核酸序列依赖性扩增技术，奥加农公司，荷兰博克斯泰尔），对来自59名HIV-1感染者的70份样本以及来自28名未感染的志愿献血者的29份样本进行HIV-1 RNA检测。HIV-1阳性血浆样本取自处于不同感染阶段的患者：两份来自有急性HIV感染体征的人（疾病控制与预防中心I期）；29份来自25名无症状者（疾病控制与预防中心II期）以及39份来自32名有艾滋病相关症状的人（疾病控制与预防中心IV期）。所有来自HIV-1感染者的样本均含有HIV-1 RNA，与感染阶段无关（敏感性100%）。对献血者样本进行重复检测，最初54/58份样本（93%）HIV-1 RNA检测呈阴性。对4份检测结果不一致的样本进行重复检测，结果显示所有样本均为阴性（特异性100%）。通过等温扩增（核酸序列依赖性扩增技术）检测血浆中的HIV-1 RNA有望成为通过聚合酶链反应检测HIV-1 RNA或DNA的一种有价值的替代方法。