Brown J M, Kaneshima H, Mocarski E S
Department of Microbiology, Stanford University School of Medicine, CA 94305-5402, USA.
J Infect Dis. 1995 Jun;171(6):1599-603. doi: 10.1093/infdis/171.6.1599.
The ability of a low-passage strain (Toledo) and laboratory strains (AD169 and Towne) of human cytomegalovirus to replicate in SCID-hu (thymus plus liver) mice were compared. At a time of peak replication, 14 days after inoculation, the Toledo strain grew 2-3 orders of magnitude better than any laboratory strain, a difference reflecting the number of infected thymic stromal cells in the implants. The growth property of the Toledo strain was stable through serial passage and plaque purification. The AD169-ATCC strain failed to grow at all, while an independently maintained stock of this strain obtained from the United Kingdom replicated to low levels, suggesting that divergence had occurred during propagation in different locations. This work predicts the existence of viral genetic determinant(s) for growth in tissues that are lost during propagation in culture.
比较了人巨细胞病毒的低传代毒株（托莱多株）和实验室毒株（AD169株和汤氏株）在SCID-hu（胸腺加肝脏）小鼠体内的复制能力。在接种后14天的复制高峰期，托莱多株的生长比任何实验室毒株都要好2至3个数量级，这种差异反映了植入物中被感染的胸腺基质细胞数量。托莱多株的生长特性通过连续传代和蚀斑纯化保持稳定。AD169-ATCC株根本无法生长，而从英国获得的该毒株的独立保存株能低水平复制，这表明在不同地点传代过程中发生了分化。这项研究预示着存在决定病毒在组织中生长的遗传决定因素，这些因素在培养传代过程中丢失了。
