Egan M A, Pavlat W A, Tartaglia J, Paoletti E, Weinhold K J, Clements M L, Siliciano R F
Department of Medicine, School of Medicine, Johns Hopkins University, Baltimore, Maryland 21205, USA.
J Infect Dis. 1995 Jun;171(6):1623-7. doi: 10.1093/infdis/171.6.1623.
CD8+ cytolytic T lymphocytes (CTL) are likely to be an important component of effective vaccines against human immunodeficiency virus type 1 (HIV-1). CTL can be induced most effectively with live virus vectors. However, because of concerns about the safety of such vectors, a nonreplicating canarypox vector (ALVAC) capable of expressing foreign genes in mammalian cells has been developed. This study evaluated the capacity of an ALVAC vector expressing the HIV-1MN envelope (env) glycoprotein to induce HIV-1-specific CTL in seronegative volunteers. Protocols were designed to determine whether immunization with ALVAC alone or in combination with subunit boosting could induce CTL in vaccinia-immune and -naive volunteers. A simple method for antigen-specific in vitro stimulation was used to detect CTL responses in HIV-1-seronegative vaccine recipients. The results indicate that low doses of a nonreplicating virus vector alone can elicit both CD4+ and CD8+ HIV-1-specific CTL in a subset of seronegative volunteers.
CD8 + 细胞毒性T淋巴细胞（CTL）可能是抗1型人类免疫缺陷病毒（HIV - 1）有效疫苗的重要组成部分。活病毒载体能最有效地诱导CTL。然而，由于担心此类载体的安全性，已开发出一种能够在哺乳动物细胞中表达外源基因的非复制型金丝雀痘病毒载体（ALVAC）。本研究评估了表达HIV - 1MN包膜（env）糖蛋白的ALVAC载体在血清阴性志愿者中诱导HIV - 1特异性CTL的能力。设计方案以确定单独使用ALVAC免疫或与亚单位加强免疫联合使用是否能在接种过痘苗和未接种过痘苗的志愿者中诱导CTL。一种简单的抗原特异性体外刺激方法用于检测HIV - 1血清阴性疫苗接种者的CTL反应。结果表明，低剂量的非复制型病毒载体单独即可在一部分血清阴性志愿者中引发CD4 + 和CD8 + HIV - 1特异性CTL。
