细胞内因子对KAT1通道电压依赖性的调节。
Regulation of voltage dependence of the KAT1 channel by intracellular factors.
作者信息
Hoshi T
机构信息
Department of Physiology and Biophysics, University of Iowa College of Medicine, Iowa City 52242, USA.
出版信息
J Gen Physiol. 1995 Mar;105(3):309-28. doi: 10.1085/jgp.105.3.309.
Abstract
The KAT1 channel is a hyperpolarization-activated K+ channel cloned from the higher plant Arabidopsis. The deduced amino acid sequence suggests that its structural organization is similar to that of the Shaker-like K+ channel activated by depolarization. Electrophysiological properties of the KAT1 channel expressed in Xenopus oocytes indicate that voltage-dependent activation of the KAT1 channel is not caused by the divalent ion block and that it is intrinsic to the channel. Activity of the KAT1 channel progressively decreases upon patch excision. This rundown of the channel is accompanied by a large shift in the voltage dependence of the channel to a more negative direction. The voltage dependence is also regulated by pH, ATP, and cGMP.
摘要
KAT1通道是从高等植物拟南芥中克隆出来的一种超极化激活的钾离子通道。推导的氨基酸序列表明，其结构组织与去极化激活的类Shaker钾离子通道相似。在非洲爪蟾卵母细胞中表达的KAT1通道的电生理特性表明，KAT1通道的电压依赖性激活不是由二价离子阻断引起的，而是通道固有的特性。KAT1通道的活性在膜片钳切除后逐渐降低。通道的这种衰减伴随着通道电压依赖性向更负方向的大幅偏移。电压依赖性也受pH、ATP和环鸟苷酸的调节。
相似文献
1
Regulation of voltage dependence of the KAT1 channel by intracellular factors.细胞内因子对KAT1通道电压依赖性的调节。
J Gen Physiol. 1995 Mar;105(3):309-28. doi: 10.1085/jgp.105.3.309.
2
Rundown of the hyperpolarization-activated KAT1 channel involves slowing of the opening transitions regulated by phosphorylation.超极化激活的KAT1通道的衰减涉及由磷酸化调节的开放转变的减慢。
Biophys J. 1999 Jun;76(6):3089-98. doi: 10.1016/S0006-3495(99)77461-8.
3
Expression of a cloned plant K+ channel in Xenopus oocytes: analysis of macroscopic currents.克隆的植物钾离子通道在非洲爪蟾卵母细胞中的表达：宏观电流分析
Plant J. 1995 Feb;7(2):321-32. doi: 10.1046/j.1365-313x.1995.7020321.x.
4
Changes in voltage activation, Cs+ sensitivity, and ion permeability in H5 mutants of the plant K+ channel KAT1.植物钾离子通道KAT1的H5突变体中电压激活、铯离子敏感性和离子通透性的变化。
Proc Natl Acad Sci U S A. 1996 Jul 23;93(15):8123-8. doi: 10.1073/pnas.93.15.8123.
5
Inward rectifier potassium channels in plants differ from their animal counterparts in response to voltage and channel modulators.植物中的内向整流钾通道在对电压和通道调节剂的反应方面与其动物对应物不同。
Eur Biophys J. 1995;24(2):107-15. doi: 10.1007/BF00211406.
6
The baculovirus/insect cell system as an alternative to Xenopus oocytes. First characterization of the AKT1 K+ channel from Arabidopsis thaliana.杆状病毒/昆虫细胞系统作为非洲爪蟾卵母细胞的替代物。来自拟南芥的AKT1钾通道的首次表征。
J Biol Chem. 1996 Sep 13;271(37):22863-70. doi: 10.1074/jbc.271.37.22863.
7
Histidine(118) in the S2-S3 linker specifically controls activation of the KAT1 channel expressed in Xenopus oocytes.S2-S3连接区中的组氨酸（118）特异性控制非洲爪蟾卵母细胞中表达的KAT1通道的激活。
Biophys J. 2000 Mar;78(3):1255-69. doi: 10.1016/S0006-3495(00)76682-3.
8
9
Level of expression in Xenopus oocytes affects some characteristics of a plant inward-rectifying voltage-gated K+ channel.非洲爪蟾卵母细胞中的表达水平会影响一种植物内向整流电压门控钾离子通道的某些特性。
Pflugers Arch. 1994 Oct;428(3-4):422-4. doi: 10.1007/BF00724528.
10
Evidence for a multi-ion pore behavior in the plant potassium channel KAT1.植物钾通道KAT1中多离子孔行为的证据。
J Membr Biol. 1998 Nov 15;166(2):91-100. doi: 10.1007/s002329900451.
引用本文的文献
1
Moonlighting Crypto-Enzymes and Domains as Ancient and Versatile Signaling Devices.兼职加密酶和域作为古老而多功能的信号装置。
Int J Mol Sci. 2024 Sep 2;25(17):9535. doi: 10.3390/ijms25179535.
2
Anisotropic Network Analysis of Open/Closed HCN4 Channel Advocates Asymmetric Subunit Cooperativity in cAMP Modulation of Gating.HCN4 通道开/闭型的各向异性网络分析提倡 cAMP 门控调节中不对称亚基协同作用。
J Chem Inf Model. 2024 Jun 24;64(12):4727-4738. doi: 10.1021/acs.jcim.4c00360. Epub 2024 Jun 3.
3
A charged existence: A century of transmembrane ion transport in plants.充满挑战的历程：一个世纪的植物跨膜离子转运。
Plant Physiol. 2024 Apr 30;195(1):79-110. doi: 10.1093/plphys/kiad630.
4
Moonlighting Proteins Shine New Light on Molecular Signaling Niches.兼职蛋白为分子信号微环境带来新见解。
Int J Mol Sci. 2021 Jan 29;22(3):1367. doi: 10.3390/ijms22031367.
5
Electromechanical coupling in the hyperpolarization-activated K channel KAT1.超极化激活钾通道 KAT1 的机电耦联。
Nature. 2020 Jul;583(7814):145-149. doi: 10.1038/s41586-020-2335-4. Epub 2020 May 27.
6
K Channel-SEC11 Binding Exchange Regulates SNARE Assembly for Secretory Traffic.K 通道-SEC11 结合交换调控分泌运输中的 SNARE 组装。
Plant Physiol. 2019 Nov;181(3):1096-1113. doi: 10.1104/pp.19.00919. Epub 2019 Sep 23.
7
The K-Uptake Permease 5 (AtKUP5) Contains a Functional Cytosolic Adenylate Cyclase Essential for K Transport.钾离子吸收通透酶5（AtKUP5）含有一个对钾离子转运至关重要的功能性胞质腺苷酸环化酶。
Front Plant Sci. 2018 Nov 13;9:1645. doi: 10.3389/fpls.2018.01645. eCollection 2018.
8
Evolution and Structural Characteristics of Plant Voltage-Gated K Channels.植物电压门控钾通道的进化与结构特征。
Plant Cell. 2018 Dec;30(12):2898-2909. doi: 10.1105/tpc.18.00523. Epub 2018 Nov 1.
9
Moonlighting Proteins and Their Role in the Control of Signaling Microenvironments, as Exemplified by cGMP and Phytosulfokine Receptor 1 (PSKR1).兼职蛋白及其在信号微环境控制中的作用，以环鸟苷酸和植物硫肽激素受体1（PSKR1）为例
Front Plant Sci. 2018 Mar 28;9:415. doi: 10.3389/fpls.2018.00415. eCollection 2018.
10
Structural insights into the mechanisms of CNBD channel function.CNBD 通道功能机制的结构见解。
J Gen Physiol. 2018 Feb 5;150(2):225-244. doi: 10.1085/jgp.201711898. Epub 2017 Dec 12.
本文引用的文献
1
Voltage dependence of K channels in guard-cell protoplasts.保卫细胞原生质体钾通道的电压依赖性。
Proc Natl Acad Sci U S A. 1987 Jun;84(12):4108-12. doi: 10.1073/pnas.84.12.4108.
2
Expression of an inward-rectifying potassium channel by the Arabidopsis KAT1 cDNA.拟南芥KAT1 cDNA对内向整流钾通道的表达
Science. 1992 Dec 4;258(5088):1654-8. doi: 10.1126/science.8966547.
3
Primary structure and functional expression of a rat G-protein-coupled muscarinic potassium channel.大鼠G蛋白偶联毒蕈碱钾通道的一级结构与功能表达
Nature. 1993 Aug 26;364(6440):802-6. doi: 10.1038/364802a0.
4
Shaker potassium channel gating. III: Evaluation of kinetic models for activation.震颤钾通道门控。III：激活动力学模型的评估。
J Gen Physiol. 1994 Feb;103(2):321-62. doi: 10.1085/jgp.103.2.321.
5
Shaker potassium channel gating. II: Transitions in the activation pathway.震颤器钾通道门控。II：激活途径中的转变。
J Gen Physiol. 1994 Feb;103(2):279-319. doi: 10.1085/jgp.103.2.279.
6
Shaker potassium channel gating. I: Transitions near the open state.摇椅式钾通道门控。I：开放状态附近的转变。
J Gen Physiol. 1994 Feb;103(2):249-78. doi: 10.1085/jgp.103.2.249.
7
Mechanism and modulation of inactivation of the Kv3 potassium channel.Kv3钾通道失活的机制与调控
Recept Channels. 1993;1(1):81-8.
8
Ether-à-go-go encodes a voltage-gated channel permeable to K+ and Ca2+ and modulated by cAMP.“激离”基因编码一种对钾离子和钙离子通透且受环磷酸腺苷调节的电压门控通道。
Nature. 1993 Sep 30;365(6445):445-8. doi: 10.1038/365445a0.
9
Primary structure and functional expression of a mouse inward rectifier potassium channel.小鼠内向整流钾通道的一级结构与功能表达
Nature. 1993 Mar 11;362(6416):127-33. doi: 10.1038/362127a0.
10
Cloning and expression of an inwardly rectifying ATP-regulated potassium channel.内向整流型ATP调节钾通道的克隆与表达
Nature. 1993 Mar 4;362(6415):31-8. doi: 10.1038/362031a0.
Zotero 插件