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一种鹿科动物DNA重复序列家族的序列特征

Sequence characteristics of a cervid DNA repeat family.

作者信息

Qureshi S A, Blake R D

机构信息

Department of Biochemistry, Microbiology, and Molecular Biology, University of Maine, Orono 04469, USA.

出版信息

J Mol Evol. 1995 Apr;40(4):400-4. doi: 10.1007/BF00164026.

DOI:10.1007/BF00164026
PMID:7769617
Abstract

The (G + C) distribution and the presence and amounts of repetitive sequence families in the white-tailed deer (Odocoileus virginianus) have been examined. The distribution ranges from 20 to 70% (G+C) and shows four distinct repeat families. A 0.7-kb family, DII, corresponds to satellite II in domestic bovids--ox, sheep, and goat--and was singled out for detailed characterization. DII has a prototypic repeat of 67% (G + C), consists of 25,000 tandem copies, and contributes 1.7% to the genomic DNA. Sequencing and electrophoretic analysis indicate a repeat length of 691 bp. These characteristics are similar to those of the bovid satellite II families as well as to those of other cervids that we have examined. The intraspecific sequence divergence within this family has a variance of only 2.5 +/- 0.3%.

摘要

已对白尾鹿(弗吉尼亚鹿)的(G + C)分布以及重复序列家族的存在情况和数量进行了研究。其分布范围为20%至70%(G + C),并显示出四个不同的重复家族。一个0.7千碱基对的家族,即DII,与家养牛科动物(牛、绵羊和山羊)中的卫星II相对应,并被挑选出来进行详细表征。DII具有67%(G + C)的原型重复序列,由25,000个串联拷贝组成,占基因组DNA的1.7%。测序和电泳分析表明重复长度为691碱基对。这些特征与牛科卫星II家族以及我们研究过的其他鹿科动物的特征相似。该家族内的种内序列差异方差仅为2.5 +/- 0.3%。

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