Letvin N L, Li J, Halloran M, Cranage M P, Rud E W, Sodroski J
Harvard Medical School, Beth Israel Hospital, Boston, Massachusetts 02215, USA.
J Virol. 1995 Jul;69(7):4569-71. doi: 10.1128/JVI.69.7.4569-4571.1995.
Prior infection with a nef-deleted simian immunodeficiency virus (SIV) protects macaques not only against a homologous pathogenic SIV challenge but also against challenge with a chimeric SIV expressing a human immunodeficiency virus type 1 env gene (SHIV). Since this SHIV is itself nonpathogenic, we sought to explore the use of a nonpathogenic SHIV as a live, attenuated AIDS virus vaccine. Four cynomolgus monkeys infected for greater than 600 days with a chimeric virus composed of SIVmac 239 expressing the human immunodeficiency virus type 1 HXBc2 env, tat, and rev genes were challenged intravenously with 100 animal infectious doses of the J5 clone of SIVmac 32H, an isolate derived by in vivo passage of SIVmac 251. Three of the four monkeys became infected with SIVmac. This observation underlines the difficulty, even with a live virus vaccine, in protecting against an AIDS virus infection.
先前感染缺失nef基因的猿猴免疫缺陷病毒（SIV）不仅能保护猕猴抵御同源致病性SIV攻击，还能抵御表达1型人类免疫缺陷病毒env基因的嵌合SIV（SHIV）的攻击。由于这种SHIV本身无致病性，我们试图探索使用无致病性的SHIV作为减毒活艾滋病病毒疫苗。四只食蟹猴感染由表达1型人类免疫缺陷病毒HXBc2 env、tat和rev基因的SIVmac 239组成的嵌合病毒超过600天，然后静脉注射100个动物感染剂量的SIVmac 32H的J5克隆（一种通过SIVmac 251体内传代获得的分离株）进行攻击。四只猴子中有三只感染了SIVmac。这一观察结果凸显了即使使用活病毒疫苗，预防艾滋病病毒感染也存在困难。
