Wu M H, Shi Q, Wechezak A R, Clowes A W, Gordon I L, Sauvage L R
Hope Heart Institute, Providence Medical Center, Seattle, WA 98122, USA.
J Vasc Surg. 1995 May;21(5):862-7. doi: 10.1016/s0741-5214(05)80019-9.
A 10 mm woven Dacron axillofemoral bypass graft was removed from a 65-year-old patient during redo surgery after an implant period of 26 months, because of a large seroma that surrounded the entire length of the graft. Tissue blocks were taken from representative areas along the entire length of the graft surface and evaluated by light microscopy with hematoxylin and eosin and Masson trichrome staining, scanning electron microscopy, transmission electron microscopy, and immunocytochemical staining. Paraffin-embedded sections were stained with smooth muscle cell alpha-actin, which demonstrated smooth muscle cells in the pseudointima, and Ham 56 stain to identify macrophages. Endothelial factor VIII/von Willebrand factor and Ulex europaeus agglutinin identified human endothelial cells on the flow surface, in areas far removed from the anastomoses to the native vessels. This is the first definitive proof in a human of endothelialization of a synthetic arterial graft beyond the pannus ingrowth zone.
在植入26个月后进行的再次手术中，从一名65岁患者身上取出了一段10毫米编织涤纶腋股旁路移植血管，原因是移植血管全长被一个巨大的血清肿包围。沿着移植血管表面的代表性区域获取组织块，并通过苏木精和伊红染色及马松三色染色的光学显微镜、扫描电子显微镜、透射电子显微镜和免疫细胞化学染色进行评估。石蜡包埋切片用平滑肌细胞α-肌动蛋白染色，显示假内膜中有平滑肌细胞，并用Ham 56染色来识别巨噬细胞。内皮因子VIII/血管性血友病因子和欧洲荆豆凝集素确定了血流表面远离与天然血管吻合处区域的人内皮细胞。这是在人类中首次明确证明合成动脉移植血管在血管翳向内生长区域之外实现了内皮化。
