Shi Q, Wu M H, Hayashida N, Wechezak A R, Clowes A W, Sauvage L R
Hope Heart Institute, University of Washington School of Medicine, Seattle 98122.
J Vasc Surg. 1994 Oct;20(4):546-56; discussion 556-7. doi: 10.1016/0741-5214(94)90279-8.
The purpose of this study was to prove directly whether cells from the blood stream contribute to endothelialization of isolated, impervious Dacron vascular grafts in the dog.
We designed an 18 cm, three-component graft with two parallel central Dacron limbs; one was made impervious with silicone rubber, and the other was preclotted. This model was implanted in the canine descending thoracic aorta with 30 microns polytetrafluoroethylene grafts anastomosed at each end. An 8 cm, three-component graft completely coated with silicone rubber was implanted in the canine abdominal aorta and inferior vena cava. Implant periods ranged from 4 to 12 weeks. Flow surfaces were studied by use of stereomicroscopy after being stained with silver nitrate, and by use of scanning and transmission electron microscopy, the inner wall and flow surface were studied by light microscopy after hematoxylin-eosin and immunocytochemical staining (the latter for endothelial and smooth muscle cells), and the full wall was studied by light microscopy after hematoxylin-eosin staining.
Effective prevention of pannus and transmural ingrowth into the impervious central test grafts was achieved, and scattered islands of endothelial cells were conclusively demonstrated on flow surfaces in each of the three implant sites 4 weeks after implantation. In the descending thoracic aorta, where these grafts were also implanted for 8 and 12 weeks, alpha-actin-positive cells and microvessels were found beneath some of the endothelial islands.
Fallout endothelialization of Dacron vascular grafts occurs in both the arterial and venous systems of the dog.
本研究的目的是直接证明血流中的细胞是否有助于犬体内孤立的、不透水的涤纶血管移植物的内皮化。
我们设计了一种18厘米长的三组件移植物，带有两个平行的中央涤纶分支；其中一个用硅橡胶制成不透水的，另一个预先凝血。该模型植入犬降主动脉，两端吻合30微米的聚四氟乙烯移植物。一个8厘米长、完全涂有硅橡胶的三组件移植物植入犬腹主动脉和下腔静脉。植入期为4至12周。用硝酸银染色后通过体视显微镜研究血流表面，通过扫描和透射电子显微镜研究内壁和血流表面，用苏木精-伊红和免疫细胞化学染色（后者用于内皮细胞和平滑肌细胞）后通过光学显微镜研究全壁，用苏木精-伊红染色后通过光学显微镜研究全壁。
有效防止了不透水的中央测试移植物中形成血管翳和透壁向内生长，并且在植入后4周，在三个植入部位的每个部位的血流表面均确凿地证实了散在的内皮细胞岛。在降主动脉中，这些移植物也植入了8周和12周，在一些内皮岛下方发现了α-肌动蛋白阳性细胞和微血管。
涤纶血管移植物的沉降内皮化发生在犬的动脉和静脉系统中。
