Ma Z Q, Zhao Y H, Sorrells M E
Department of Plant Breeding and Biometry, Cornell University, Ithaca, NY 14853, USA.
Mol Gen Genet. 1995 May 10;247(3):351-7. doi: 10.1007/BF00293203.
Restriction fragment length polymorphism (RFLP) markers were used to map male fertility restoring gene that was transferred from chromosome 6U of Aegilops umbellulata Zhuk. to wheat. Segments of chromosome 6U bearing the gene that restore fertility to T. timopheevi Zhuk. male sterile cytoplasm were identified in all four translocation lines by two probes, BCD21 and BCD342. Lines 040-5, 061-1 and 061-4 are T6BL.6BS-6U translocations, while line 2114 is a T6AL.6AS-6U translocation. Line 2114 has a much larger 6U chromosomal segment and lower frequency of transmission of male gametes with the alien segment than the other three lines. The restoring gene carried by the 6U segment in 2114 showed high expressivity and complete penetrance. This restoring gene is designated Rf6. A homoeologous chromosome recombination mechanism is discussed for the alien gene transfer.
利用限制性片段长度多态性（RFLP）标记对从小伞山羊草（Aegilops umbellulata Zhuk.）6U染色体转移到小麦中的雄性育性恢复基因进行定位。通过两个探针BCD21和BCD342，在所有四个易位系中鉴定出携带恢复提莫菲维小麦（T. timopheevi Zhuk.）雄性不育细胞质育性基因的6U染色体片段。040 - 5、061 - 1和061 - 4系是T6BL.6BS - 6U易位系，而2114系是T6AL.6AS - 6U易位系。2114系具有大得多的6U染色体片段，并且与其他三个系相比，携带外源片段的雄配子传递频率较低。2114系中6U片段携带的恢复基因表现出高表达率和完全外显率。该恢复基因被命名为Rf6。本文还讨论了外源基因转移的同源染色体重组机制。
