Carrington W A, Lynch R M, Moore E D, Isenberg G, Fogarty K E, Fay F S
Program in Molecular Medicine, University of Massachusetts Medical School, Worcester 01605, USA.
Science. 1995 Jun 9;268(5216):1483-7. doi: 10.1126/science.7770772.
Fluorescent probes offer insight into the highly localized and rapid molecular events that underlie cell function. However, methods are required that can efficiently transform the limited signals from such probes into high-resolution images. An algorithm has now been developed that produces highly accurate images of fluorescent probe distribution inside cells with minimal light exposure and a conventional light microscope. This method provides resolution nearly four times greater than that currently available from any fluorescence microscope and was used to study several biological problems.
荧光探针有助于深入了解构成细胞功能基础的高度局部化且快速的分子事件。然而，需要一些方法能够有效地将来自此类探针的有限信号转化为高分辨率图像。现在已经开发出一种算法，该算法使用常规光学显微镜，在最小化光暴露的情况下生成细胞内荧光探针分布的高精度图像。这种方法提供的分辨率几乎比目前任何荧光显微镜所能达到的分辨率大四倍，并且已被用于研究几个生物学问题。
