Caring for your hybridization membranes.

Methods and reagents is a unique monthly column that highlights current discussions in the newsgroup bionet.molbio.methds-reagnts, available on the Internet. This month's column discusses the time limitation for using nylon-based and nitrocellulose membranes for Southern hybridizations, and there are a few other helpful hints. For details on how to partake in the newsgroup, see the accompanying box.