Davis F M, Busch R K, Yeoman L C, Busch H
Cancer Res. 1978 Jul;38(7):1906-15.
Antisera to nucleoli of Novikoff hepatoma ascites and normal rat liver cells were produced in rabbits by injection of whole, isolated nucleoli. These antisera have been used to compare the nucleolar antigens that were partially fractionated by differential solubilization from nucleoli. Fourteen antigens were detected by these antisera; ten of these antigens were detected by both antisera. Ouchterlony double diffusion analysis of soluble extracts from normal rat liver and Novikoff hepatoma ascites nucleoli and fetal rat liver nuclei provided evidence for antigens found only in liver extracts, only in tumor extracts, or only in tumor and fetal extracts. Antisera preabsorbed to remove antibodies to common antigens of liver and tumor provided confirmatory evidence for one nucleolar antigen in liver that was not found in tumor or fetal rat liver, one antigen in tumor that was not found in adult or fetal rat liver, and three antigens in both tumor and fetal rat liver that were not found in adult rat liver. In addition, the antitumor nucleolar antiserum preabsorbed with liver nuclear extracts still produced positive nucleolar fluorescence in Novikoff hepatoma ascites cells but not in liver cells. Conversely, anti-liver nucleolar antiserum preabsorbed with tumor nucleolar extracts did not produce detectable tumor nucleolar fluorescence but did produce positive fluorescence in liver nucleoli.
通过注射完整的、分离的核仁，在兔体内制备了针对诺维科夫肝癌腹水细胞和正常大鼠肝细胞核仁的抗血清。这些抗血清已被用于比较通过从核仁中进行差异溶解而部分分离得到的核仁抗原。这些抗血清检测到了14种抗原；其中10种抗原可被两种抗血清均检测到。对正常大鼠肝脏、诺维科夫肝癌腹水细胞核仁和胎鼠肝细胞核的可溶性提取物进行的双向免疫扩散分析，为仅在肝脏提取物中、仅在肿瘤提取物中或仅在肿瘤和胎儿提取物中发现的抗原提供了证据。预先吸收以去除针对肝脏和肿瘤共同抗原的抗体的抗血清，为在肝脏中发现但在肿瘤或胎鼠肝脏中未发现的一种核仁抗原、在肿瘤中发现但在成年或胎鼠肝脏中未发现的一种抗原以及在肿瘤和胎鼠肝脏中均发现但在成年大鼠肝脏中未发现的三种抗原提供了确证。此外，用肝细胞核提取物预先吸收的抗肿瘤核仁抗血清，在诺维科夫肝癌腹水细胞中仍产生阳性核仁荧光，但在肝细胞中不产生。相反，用肿瘤核仁提取物预先吸收的抗肝脏核仁抗血清，未产生可检测到的肿瘤核仁荧光，但在肝脏核仁中产生阳性荧光。
