Satoh K, Busch H
Cancer Res. 1983 May;43(5):2143-9.
Nucleolar antigens of normal rat liver, regenerating liver, and Novikoff ascites hepatoma cells were transplanted in vitro from polyadenylic acid-containing messenger RNAs isolated from the respective tissues and immunoprecipitated with specific antinucleolar antibodies and Protein A. By two-dimensional gel electrophoresis of the translation products, five antigens were detected in normal rat liver. The antigens detected in 18-hr regenerating rat liver were the same as those of normal rat liver when immunoprecipitated with the anti-liver nucleolar antibodies. In the Novikoff tumor, 11 antigens were detected with anti-Novikoff nucleolar antibodies. Of these, two were not found in either normal or regenerating liver. Four major antigens were detectable in both Novikoff hepatoma and regenerating liver messenger RNA translation products with anti-Novikoff nucleolar antibodies. Two antigens were found in normal and regenerating liver that were not found in Novikoff hepatoma. In agreement with previous results, these immunoprecipitation analyses provide further evidence that some nucleolar antigens are present in Novikoff hepatoma that are not found in either normal or regenerating rat liver.
从正常大鼠肝脏、再生肝脏以及诺维科夫腹水肝癌细胞中提取含多聚腺苷酸的信使核糖核酸，在体外进行移植，并用特异性抗核仁抗体和蛋白A进行免疫沉淀，从而分析其核仁抗原。通过对翻译产物进行二维凝胶电泳，在正常大鼠肝脏中检测到了五种抗原。当用抗肝脏核仁抗体进行免疫沉淀时，在18小时再生大鼠肝脏中检测到的抗原与正常大鼠肝脏中的相同。在诺维科夫肿瘤中，用抗诺维科夫核仁抗体检测到了11种抗原。其中，有两种在正常肝脏或再生肝脏中均未发现。用抗诺维科夫核仁抗体在诺维科夫肝癌和再生肝脏信使核糖核酸翻译产物中均可检测到四种主要抗原。在正常肝脏和再生肝脏中发现了两种在诺维科夫肝癌中未发现的抗原。与之前的结果一致，这些免疫沉淀分析进一步证明，诺维科夫肝癌中存在一些在正常大鼠肝脏或再生大鼠肝脏中均未发现的核仁抗原。
