Wen S, Alexander H, Inchikel A, Stevenson W T
Department of Chemistry, Wichita State University, KS 67260-0051, USA.
Biomaterials. 1995 Mar;16(4):325-35. doi: 10.1016/0142-9612(95)93261-b.
Methacrylic acid (MAA) based polyelectrolytes were complexed with protonated or quaternized dimethylaminoethyl methacrylate (DMAEMA) containing polyelectrolytes to form microcapsules in vitro. Anchorage independent human Burkitt lymphoma (Raji) cells were successfully cultured in the presence of dissolved MAA containing polymer. Capsule morphology was investigated by light microscopy and by scanning electron microscopy (SEM). Capsules based on quaternized DMAEMA containing polymer were found to be more stable than capsules containing protonated DMAEMA functionality. Raji cells were successfully encapsulated in both systems and divided to confluence; thereafter sufficient pressure was exerted to burst open the capsules. Cells released from these capsules appeared to suffer no discernible trauma and were successfully isolated and subcultured to confluence.
基于甲基丙烯酸（MAA）的聚电解质与含有质子化或季铵化甲基丙烯酸二甲氨基乙酯（DMAEMA）的聚电解质复合，以在体外形成微胶囊。在含有溶解的含MAA聚合物的情况下，成功培养了非贴壁依赖性人伯基特淋巴瘤（Raji）细胞。通过光学显微镜和扫描电子显微镜（SEM）研究胶囊形态。发现基于含季铵化DMAEMA聚合物的胶囊比含有质子化DMAEMA官能团的胶囊更稳定。Raji细胞在这两种系统中均成功包封并分裂至汇合；此后施加足够的压力使胶囊破裂。从这些胶囊中释放的细胞似乎没有受到明显的损伤，并成功分离并传代培养至汇合。
