Shao W, Yin X, Stevenson W T, Alexander H
Department of Chemistry, Wichita State University, KS 67208.
Biomaterials. 1991 Jul;12(5):479-88. doi: 10.1016/0142-9612(91)90146-2.
Relationships between ionizable group content, structure and molecular weight, solubility and solution behaviour, and the efficacy of ionic complex formation through complex coacervation, have been established for a range of sparingly soluble synthetic weak polyelectrolyte polymers with low charge content, based on hydroxyalkyl methacrylates. Selected polymers containing methacrylic acid (acidic) and dimethylaminoethyl methacrylate (basic) functionality show promise as capsule-forming pairs for the entrapment of mammalian cells. The solubility of basic polymers and their ability to form microcapsules with structural integrity is enhanced through quaternization of the N-methyl functionality to the quaternary ammonium group. The survival of guinea-pig erythrocytes encapsulated for 4 d in this promising system was shown by the Drabkin's test to be about 41% of the control sample.
对于一系列基于甲基丙烯酸羟烷基酯的、具有低电荷含量的微溶性合成弱聚电解质聚合物，已经确定了其可电离基团含量、结构与分子量、溶解度和溶液行为之间的关系，以及通过复凝聚形成离子复合物的功效。含有甲基丙烯酸（酸性）和甲基丙烯酸二甲氨基乙酯（碱性）官能团的选定聚合物显示出有望成为用于包裹哺乳动物细胞的成囊对。通过将N-甲基官能团季铵化为季铵基团，提高了碱性聚合物的溶解度及其形成具有结构完整性的微胶囊的能力。通过德拉布金氏试验表明，在这个有前景的系统中封装4天的豚鼠红细胞存活率约为对照样品的41%。
