Wegmann R, Balmain N, Ricard-Blum S, Guha S
Universités de Paris, France.
Cell Mol Biol (Noisy-le-grand). 1995 Feb;41(1):1-20.
A general survey is presented on the most important applications of radioactive compounds as well on fresh as on fixed tissues, with and without immunological reactions, at light and electron microscopic levels. Its goal is to show their flexibility and their extended applications, in comparison with the non-radioactive methods. But radioautography applies as well to non-cellular aspects, such as electrophoretic and chromatographic techniques, permitting a complementary and even more detailed exploration of the molecules investigated at cellular levels. A rapid information is given on the exact denomination of the radioautographic methods, on hybridization in situ and in vitro, on the different blotting techniques used for DNA, RNA and proteins, on semi-quantitation and quantitation of DNA-RNA hybrids, on radioimmunodection by fluorography and on newer filmless radioautographic systems. The organ, body and pharmacological radioautographies belong to the nuclear medicine and have been evocated briefly.
本文综述了放射性化合物在新鲜组织和固定组织中的最重要应用，包括有无免疫反应，以及在光学显微镜和电子显微镜水平上的应用。其目的是展示与非放射性方法相比，它们的灵活性和广泛应用。但放射自显影也适用于非细胞方面，如电泳和色谱技术，从而能够对细胞水平上研究的分子进行补充且更详细的探索。本文快速介绍了放射自显影方法的确切名称、原位和体外杂交、用于DNA、RNA和蛋白质的不同印迹技术、DNA-RNA杂交体的半定量和定量、荧光自显影放射免疫检测以及更新的无胶片放射自显影系统。器官、身体和药理放射自显影属于核医学，在此进行了简要介绍。
