Swindells M B
Protein Engineering Research Institute, Osaka, Japan.
Protein Sci. 1995 Jan;4(1):103-12. doi: 10.1002/pro.5560040113.
A procedure is described for detecting domains in proteins of known structure. The method is based on the intuitively simple idea that each domain should contain an identifiable hydrophobic core. By applying the algorithm described in the companion paper (Swindells MB, 1995, Protein Sci 4:93-102) to identify distinct cores in multi-domain proteins, one can use this information to determine both the number and the location of the constituent domains. Tests have shown the procedure to be effective on a number of examples, even when the domains are discontinuous along the sequence. However, deficiencies also occur when hydrophobic cores from different domains continue through the interface region and join one another.
本文描述了一种用于检测已知结构蛋白质中结构域的方法。该方法基于一个直观简单的理念，即每个结构域都应包含一个可识别的疏水核心。通过应用配套论文（Swindells MB，1995，Protein Sci 4：93 - 102）中描述的算法来识别多结构域蛋白质中的不同核心，人们可以利用这些信息来确定组成结构域的数量和位置。测试表明，该方法在多个实例中均有效，即使结构域在序列上是不连续的。然而，当来自不同结构域的疏水核心穿过界面区域并相互连接时，也会出现不足之处。
