环磷酸腺苷（cAMP）对丝裂原刺激的B淋巴细胞碱性磷酸酶活性和增殖的正向调节作用。

Positive regulatory role of cAMP on alkaline phosphatase activity and proliferation of mitogen stimulated B lymphocytes.

作者信息

Kasyapa C S, Ramanadham M

机构信息

Department of Biochemistry, School of Life Sciences University of Hyderabad, India.

出版信息

Biochem Mol Biol Int. 1995 Mar;35(3):535-40.

PMID:7773190

Abstract

Effect of dibutyryl cyclic adenosine monophosphate (dbt cAMP) on alkaline phosphatase (APase) of mitogen stimulated murine B lymphocytes was studied. Addition of dbtcAMP to lipopolysaccharide (LPS) stimulated B cells enhanced APase activity in a dose dependent and synergistic manner. dbtcAMP also stimulated the proliferative response of LPS treated B lymphocytes. On the other hand, when B lymphocytes stimulated with anti-immunoglobulin (anti-Ig) were treated with dbtcAMP neither DNA synthesis nor APase activity was enhanced. These results suggest that cAMP is a potent synergistic activator of APase in B lymphocytes committed to proliferation.

摘要

研究了二丁酰环磷腺苷（dbt cAMP）对丝裂原刺激的小鼠B淋巴细胞碱性磷酸酶（APase）的影响。将dbtcAMP添加到脂多糖（LPS）刺激的B细胞中，以剂量依赖性和协同方式增强了APase活性。dbtcAMP还刺激了LPS处理的B淋巴细胞的增殖反应。另一方面，当用抗免疫球蛋白（抗Ig）刺激的B淋巴细胞用dbtcAMP处理时，DNA合成和APase活性均未增强。这些结果表明，cAMP是致力于增殖的B淋巴细胞中APase的有效协同激活剂。