Bukreyev A A, Belanov E F, Blinov V M, Netesov S V
State Research Center of Virology and Biotechnology Vector, Institute of Molecular Biology, Koltsovo, Novosibirsk Region, Russia.
Biochem Mol Biol Int. 1995 Mar;35(3):605-13.
Nucleotide sequences of the genes 5 and 6 of the Marburg virus, Popp strain, were determined. ORFs encoding polypeptides VP30 (281 a.a., MW 32,640) and VP24 (253 a.a., MW 28,621) were found. The putative transcription start and stop signals for viral RNA-dependent RNA polymerase were revealed for both genes. Overlapping of genes 5 and 6 was shown. The deduced amino acid sequences of VP30 and VP24 proteins displayed significant homology with the analogous proteins of another filovirus, the Ebola virus (33% and 37%, respectively). The VP24 appeared to have a hydrophobic amino acid composition; content of hydrophobic amino acids was 40.7%. Model of VP24 location in the virion was suggested.
确定了马尔堡病毒波普毒株基因5和基因6的核苷酸序列。发现了编码多肽VP30（281个氨基酸，分子量32,640）和VP24（253个氨基酸，分子量28,621）的开放阅读框。揭示了这两个基因的病毒RNA依赖性RNA聚合酶的推定转录起始和终止信号。显示了基因5和基因6的重叠。推导的VP30和VP24蛋白的氨基酸序列与另一种丝状病毒埃博拉病毒的类似蛋白具有显著同源性（分别为33%和37%）。VP24似乎具有疏水氨基酸组成；疏水氨基酸含量为40.7%。提出了VP24在病毒粒子中的定位模型。
