Ihan A, Krizman I, Ferlan-Marolt V, Tepez B, Gubina M
Institute of Microbiology, Medical Faculty, Ljubljana, Slovenia.
FEMS Immunol Med Microbiol. 1995 Feb;10(3-4):295-9. doi: 10.1111/j.1574-695X.1995.tb00047.x.
We isolated lymphocytes from chronically inflamed gastric mucosa. We analysed the expression of IL-2 receptors (CD25), transferin receptors (CD71) and HLA-DR molecules on T lymphocytes by flow cytometric analysis in 16 patients with urease-positive and in 7 patients with urease-negative chronic gastritis. In G0, G1 and G2 histological type (Sydney classification) of gastritis the number of lymphocytes obtained from the gastric mucosa biopsies was too low for the flow cytometric analysis. However, in G3 histological type of chronic gastritis we obtained enough cells for the flow cytometric analysis in 75%. We demonstrated a significant increase in HLA-DR expression on CD8 cells from patients with urease-positive gastritis compared to urease-negative gastritis. We also observed a statistically non-significant increase in HLA-DR expression on CD3 cells, and in CD71 expression on both CD3 and CD8 cells in urease-positive gastritis. However, no difference in CD25 expression was found between the two types of gastritis.
我们从慢性炎症性胃黏膜中分离出淋巴细胞。通过流式细胞术分析，我们检测了16例尿素酶阳性和7例尿素酶阴性慢性胃炎患者T淋巴细胞上白细胞介素-2受体（CD25）、转铁蛋白受体（CD71）和HLA-DR分子的表达。在胃炎的G0、G1和G2组织学类型（悉尼分类法）中，从胃黏膜活检获得的淋巴细胞数量过少，无法进行流式细胞术分析。然而，在G3组织学类型的慢性胃炎中，75%的样本获得了足够用于流式细胞术分析的细胞。我们发现，与尿素酶阴性胃炎患者相比，尿素酶阳性胃炎患者CD8细胞上的HLA-DR表达显著增加。我们还观察到，在尿素酶阳性胃炎中，CD3细胞上的HLA-DR表达以及CD3和CD8细胞上的CD71表达虽有增加，但无统计学意义。然而，两种类型的胃炎在CD25表达上未发现差异。
