Marshall D G, Chua A, Keeling P W, Sullivan D J, Coleman D C, Smyth C J
University of Dublin, Moyne Institute, Department of Microbiology, Trinity College, Ireland.
FEMS Immunol Med Microbiol. 1995 Feb;10(3-4):317-23. doi: 10.1111/j.1574-695X.1995.tb00050.x.
In the present study, randomly amplified polymorphic DNA (RAPD) fingerprinting has been used to analyse multiple single colony isolates of Helicobacter pylori from antral biopsies in an attempt to ascertain whether or not multiple strains are present in individual patients using single biopsy samples. The RAPD fingerprints derived from single colonies obtained from the same biopsy specimen were in all cases indistinguishable. The previously noted heterogeneity between H. pylori strains from different individuals was confirmed. RAPD fingerprinting, combined with a simple method of template preparation, was shown to be an excellent method for H. pylori strain differentiation. The results of this study indicate that the H. pylori population is homogeneous in individual patients at a single gastric site.
在本研究中，随机扩增多态性DNA（RAPD）指纹图谱已被用于分析来自胃窦活检的幽门螺杆菌多个单菌落分离株，以试图确定使用单个活检样本的个体患者中是否存在多种菌株。从同一活检标本获得的单菌落产生的RAPD指纹图谱在所有情况下均无法区分。先前观察到的不同个体的幽门螺杆菌菌株之间的异质性得到了证实。RAPD指纹图谱与一种简单的模板制备方法相结合，被证明是区分幽门螺杆菌菌株的一种优秀方法。本研究结果表明，在单个胃部位的个体患者中，幽门螺杆菌群体是同质的。
