DiRuggiero J, Tuttle J H, Robb F T
Center of Marine Biotechnology, University of Maryland System, Baltimore 21202, USA.
Mol Mar Biol Biotechnol. 1995 Jun;4(2):123-7.
We report a sensitive and convenient method for rapid differentiation of new isolates of hyperthermophilic Archaea. Polymerase chain reaction (PCR) was used to amplify the intergenic spacer regions of the ribosomal RNA operons of eight Archaea. Spacer regions from one Euryarcheote, Pyrococcus furiosus, and one Crenarcheote, Pyrodictium brockii, were sequenced completely. Restriction fragment length polymorphism (RFLP) analyses were performed on the spacer regions from eight hyperthermophilic Archaea, and the restriction patterns were used as fingerprints for six known strains and two isolates. The PCR-RFLP method used in this study allowed the differentiation of seven of the eight strains tested and could be generally applicable to all the Archaea.
我们报告了一种灵敏且便捷的方法，用于快速鉴别嗜热古菌的新分离株。采用聚合酶链反应（PCR）扩增8种古菌核糖体RNA操纵子的基因间间隔区。对来自广古菌门的激烈火球菌和泉古菌门的布氏火盘菌的间隔区进行了全序列测定。对8种嗜热古菌的间隔区进行了限制性片段长度多态性（RFLP）分析，所得限制性图谱用作6株已知菌株和2株分离株的指纹图谱。本研究中使用的PCR-RFLP方法能够鉴别所检测的8株菌株中的7株，并且通常适用于所有古菌。
